RHE, gene expression (cytokine stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. DEFB4, S100A7, CXCL8, KRT10,..., mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 |
| EPIBA-0006 | Psoriasis, Psoriasis | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. DEFB4, S100A7, CXCL8, KRT10,..., mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 | RT-qPCR, qPCRarray (standard or customized) | IL-17 / OSM / TNF- alpha | |
RHE, gene expression (T cell medium stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. DEFB4, S100A7, CXCL8, KRT10,..., mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 |
| EPIBA-0008 | Psoriasis, Psoriasis | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. DEFB4, S100A7, CXCL8, KRT10,..., mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 | RT-qPCR, qPCRarray (standard or customized) | Psoriatic T cell medium | |
NHEK, gene expression (T cell medium stimulation)| Type of assay | Gene expression | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | mRNA: e.g. DEFB4, S100A7, CXCL8, KRT10,..., mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 |
| NHEK-0008 | Psoriasis, Psoriasis | Gene expression | Epidermal keratinocytes (NHEK) | Human | mRNA: e.g. DEFB4, S100A7, CXCL8, KRT10,..., mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 | RT-qPCR, qPCRarray (standard or customized) | Psoriatic T cell medium | |
RHE, gene expression + histology (OSM stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. S100A7, KRT10 + histology |
| EPIBA-0005 | Psoriasis, Psoriasis | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. S100A7, KRT10 + histology | RT-qPCR + HE staining | OSM | |
RHE, gene expression + histology (cytokine stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Specific mRNA(s) + histology, mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 |
| EPIBA-0007 | Psoriasis, Psoriasis | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Specific mRNA(s) + histology, mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 | RT-qPCR + HE staining, qPCRarray (standard or customized) + HE staining | IL-17 / OSM / TNF- alpha | |
RHE, gene expression + histology (T cell medium stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Specific mRNA(s) + histology, mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 |
| EPIBA-0009 | Psoriasis, Psoriasis | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Specific mRNA(s) + histology, mQPA-NHEK-PSO-16, mQPA-NHEK-PSO-32, mQPA-NHEK-PSO-64 | RT-qPCR + HE staining, qPCRarray (standard or customized) + HE staining | Psoriatic T cell medium | |
