Online Catalogs
EPIBA-0025 - RHE, total lipids & ceramide neosynthesis (Vitamin C stimulation)
| Type of assay | Lipid synthesis |
| Biological model | Reconstructed Epidermis, BIOalternatives RHE |
| Species | Human |
| Endpoint | Total lipid & ceramide neosynthesis |
| Method | [14C]-acetate incorporation in polar lipids + TLC |
| Stimulating factor / inducer | Vitamin C |
| Reference compound | Retinoic acid |
| Physio-pathology | Differentiation, protection, barrier function |
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Reference | Physio-pathology | Type of assay | Biological model | Species | Endpoint | Method | Stimulating factor / inducer | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 RHE, total lipid & ceramide neosynthesis (basal)
| EPIBA-0024 | Differentiation, protection, barrier function | Lipid synthesis | Reconstructed Epidermis, BIOalternatives RHE | Human | Total lipid & ceramide neosynthesis | [14C]-acetate incorporation in polar lipids + TLC | Basal | |||||||||
 Ceramidase inhibition (enzyme extract)
| BIOCH-0020 | Enzyme activity assays, Differentiation, protection, barrier function | Lipid synthesis | Acellular | Ceramidase activity (basic and acid form) | [14C]-C16-ceramide degradation | |||||||||||
 NHEK, total lipid neosynthesis (basal)
| NHEK-0034 | Differentiation, protection, barrier function | Lipid synthesis | Epidermal keratinocytes (NHEK) | Human | Total lipid neosynthesis | [14C]-acetate incorporation | Basal | |||||||||
 NHEK, total lipid neosynthesis (basal)
| NHEK-0064 | Differentiation, protection, barrier function | Lipid synthesis | Epidermal keratinocytes (NHEK) | Human | Total lipid neosynthesis | [14C]-acetate incorporation in polar lipids + TLC | Basal |
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