HMSC, alkaline phosphatase activity (basal)| Type of assay | Differentiation | | Biological model | Mesenchymal stem cells (hMSC) | | Species | Human | | Endpoint | Alkaline phosphatase |
| HMSC-0003 | Osteoblast physiology | Differentiation | Mesenchymal stem cells (hMSC) | Human | Alkaline phosphatase | Fluorescence | Basal | |
Osteoblasts, proliferation (basal)| Type of assay | Proliferation | | Biological model | Osteoblasts (HOB) | | Species | Human | | Endpoint | Proliferation |
| HOB-0005 | Osteoblast physiology | Proliferation | Osteoblasts (HOB) | Human | Proliferation | [3H]-thymidine incorporation | Basal | |
MC3T3-E1, proliferation (basal)| Type of assay | Proliferation | | Biological model | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | | Species | Mouse | | Endpoint | Proliferation |
| MC3T3-0001 | Osteoblast physiology | Proliferation | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | Mouse | Proliferation | Fluorescence / imaging | Basal | |
MC3T3-E1, calcium deposition (basal)| Type of assay | Mineralization | | Biological model | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | | Species | Mouse | | Endpoint | Calcium deposition |
| MC3T3-0002 | Bone synthesis | Mineralization | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | Mouse | Calcium deposition | Photometry | Basal | |
MC3T3-E1, alkaline phosphatase activity (basal)| Type of assay | Differentiation | | Biological model | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | | Species | Mouse | | Endpoint | Alkaline phosphatase |
| MC3T3-0003 | Osteoblast physiology | Differentiation | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | Mouse | Alkaline phosphatase | Fluorescence | Basal | |
MC3T3-E1, proliferation (basal)| Type of assay | Proliferation | | Biological model | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | | Species | Mouse | | Endpoint | Proliferation |
| MC3T3-0004 | Osteoblast physiology | Proliferation | MC3T3-E1 Subclone 4 (osteoblast-like cell line) | Mouse | Proliferation | BrdU incorporation / Photometry | Basal | |
Chondrocytes, proliferation (basal)| Type of assay | Proliferation | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | Proliferation |
| NHC-0001 | Chondrocyte physiology | Proliferation | Chondrocytes (HCH) | Human | Proliferation | [3H]-thymidine incorporation | Basal | |
Chondrocytes, prolin-rich ECM proteins (basal)| Type of assay | ECM synthesis | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | Prolin-rich ECM protein synthesis |
| NHC-0002 | Cartilage synthesis | ECM synthesis | Chondrocytes (HCH) | Human | Prolin-rich ECM protein synthesis | [3H]-prolin incorporation | Basal | |
Chondrocytes, total GAGs synthesis (basal)| Type of assay | ECM synthesis | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | Glycosaminoglycans (GAGs, total) |
| NHC-0003 | Cartilage synthesis | ECM synthesis | Chondrocytes (HCH) | Human | Glycosaminoglycans (GAGs, total) | [3H]-glucosamine incorporation | Basal | |
Chondrocytes, inflammation gene expression (cytokine stimulation)| Type of assay | Gene expression | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | |
| NHC-0006 | Chondrocyte inflammation | Gene expression | Chondrocytes (HCH) | Human | | RT-qPCR | IL-1 beta / TNF- alpha / IFN- gamma / IL-6 | |
Chondrocytes, IL-8 release (cytokine stimulation)| Type of assay | Inflammatory cytokines | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | IL-8 release |
| NHC-0007 | Chondrocyte inflammation | Inflammatory cytokines | Chondrocytes (HCH) | Human | IL-8 release | ELISA / EIA | IL-1 beta / TNF- alpha / IFN- gamma / IL-6 | |
Chondrocytes, calcium deposition (basal)| Type of assay | Mineralization | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | Calcium deposition |
| NHC-0008 | Cartilage synthesis | Mineralization | Chondrocytes (HCH) | Human | Calcium deposition | Photometry, kinetic assay | | |
Chondrocytes, PGE2 release (cytokine stimulation)| Type of assay | Inflammatory cytokines | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | PGE2 release |
| NHC-0014 | Chondrocyte inflammation | Inflammatory cytokines | Chondrocytes (HCH) | Human | PGE2 release | ELISA / EIA | IL-1 beta / TNF- alpha / IFN- gamma / IL-6 | |
Chondrocytes, MMP-3 release (cytokine stimulation)| Type of assay | Inflammatory cytokines | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | MMP-3 release |
| NHC-0015 | Chondrocyte inflammation | Inflammatory cytokines | Chondrocytes (HCH) | Human | MMP-3 release | ELISA / EIA | IL-1 beta / TNF- alpha / IFN- gamma / IL-6 | |
Chondrocytes, MMP-3 release (PMA stimulation)| Type of assay | Inflammatory cytokines | | Biological model | Chondrocytes (HCH) | | Species | Human | | Endpoint | MMP-3 release |
| NHC-0018 | Chondrocyte inflammation | Inflammatory cytokines | Chondrocytes (HCH) | Human | MMP-3 release | ELISA / EIA | PMA | |
