NHEM, tyrosinase activity (basal)| Type of assay | Melanogenic proteins | | Biological model | Epidermal melanocytes (NHEM) | | Species | Human | | Endpoint | Tyrosinase activity |
| NHEM-0007 | Melanogenesis, Pigmentation disorders | Melanogenic proteins | Epidermal melanocytes (NHEM) | Human | Tyrosinase activity | Photometry | Basal | |
NHEM, pigmentation-related gene expression (basal)| Type of assay | Gene expression | | Biological model | Epidermal melanocytes (NHEM) | | Species | Human | | Endpoint | mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-NHEM-16, mQPA-NHEM-64 |
| NHEM-0009 | Melanogenesis, Pigmentation disorders | Gene expression | Epidermal melanocytes (NHEM) | Human | mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-NHEM-16, mQPA-NHEM-64 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
NHEM, proliferation (basal)| Type of assay | Proliferation | | Biological model | Epidermal melanocytes (NHEM) | | Species | Human | | Endpoint | Proliferation |
| NHEM-0016 | Melanogenesis, Pigmentation disorders | Proliferation | Epidermal melanocytes (NHEM) | Human | Proliferation | BrdU incorporation / Photometry | Basal | |
RHEm, tyrosinase expression (basal)| Type of assay | Melanogenic proteins | | Biological model | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | | Species | Human | | Endpoint | Tyrosinase immunolabeling |
| RHEM-0003 | Melanogenesis, Pigmentation disorders | Melanogenic proteins | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | Human | Tyrosinase immunolabeling | IHC | Basal | |
RHEm, TRP-1 expression (basal)| Type of assay | Melanogenic proteins | | Biological model | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | | Species | Human | | Endpoint | TRP-1 immunolabeling |
| RHEM-0005 | Melanogenesis, Pigmentation disorders | Melanogenic proteins | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | Human | TRP-1 immunolabeling | Immunohistofluorescence | Basal | |
RHEm, in situ tyrosinase activity (basal)| Type of assay | Melanogenic proteins | | Biological model | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | | Species | Human | | Endpoint | Tyrosinase activity |
| RHEM-0007 | Melanogenesis, Pigmentation disorders | Melanogenic proteins | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | Human | Tyrosinase activity | Immunohistofluorescence | Basal | |
RHEm, pigmentation-related gene expression (basal)| Type of assay | Gene expression | | Biological model | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | | Species | Human | | Endpoint | mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., qPCRarray (16 genes) |
| RHEM-0008 | Melanogenesis, Pigmentation disorders | Gene expression | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | Human | mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., qPCRarray (16 genes) | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHEm, modulation of melanin synthesis (basal)| Type of assay | Melanin synthesis | | Biological model | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | | Species | Human | | Endpoint | Melanin content |
| RHEM-0014 | Melanogenesis, Pigmentation disorders | Melanin synthesis | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | Human | Melanin content | Photometry | Basal | |
RHEm, pigmentation (Fontana-Masson staining)| Type of assay | Melanin synthesis | | Biological model | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | | Species | Human | | Endpoint | Melanin staining |
| RHEM-0015 | Melanogenesis, Pigmentation disorders | Melanin synthesis | Melanocyte-containing reconstructed epidermis, BIOalternatives RHEm | Human | Melanin staining | Histology (Fontana-Masson staining) | Basal | |
Skin maintained ex vivo, TRP-1 expression (basal)| Type of assay | Melanogenic proteins | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | TRP-1 immunolabeling |
| SKIN-0014 | Melanogenesis, Pigmentation disorders | Melanogenic proteins | Skin (ex vivo) | Human | TRP-1 immunolabeling | Immunohistofluorescence | Basal | |
Skin maintained ex vivo, pigmentation (Fontana-Masson staining)| Type of assay | Melanin synthesis | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Melanin staining |
| SKIN-0020 | Melanogenesis, Skin morphology, Pigmentation disorders | Melanin synthesis | Skin (ex vivo) | Human | Melanin staining | Histology (Fontana-Masson staining) | Basal | |
Skin maintained ex vivo, in situ tyrosinase activity (basal)| Type of assay | Melanogenic proteins | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Tyrosinase activity |
| SKIN-0024 | Melanogenesis, Pigmentation disorders | Melanogenic proteins | Skin (ex vivo) | Human | Tyrosinase activity | In situ fluorescence | Basal | |
