RHE, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | 5- alpha reductase activity |
| EPIBA-0052 | Hair growth & degeneration, Acne, Acne | Androgen metabolism | Reconstructed Epidermis, BIOalternatives RHE | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
NHDF, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Dermal fibroblasts (NHDF) | | Species | Human | | Endpoint | 5- alpha reductase activity |
| NHDF-0050 | Hair growth & degeneration, Acne, Acne | Androgen metabolism | Dermal fibroblasts (NHDF) | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
NHEK, antimicrobial peptide release (basal)| Type of assay | Skin innate defense | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | Elafin release, beta-defensin 2 release |
| NHEK-0035 | Protection against infections, skin innate immunity, Atopic dermatitis, Acne, Wound healing, trauma & agressions, Wound healing, trauma & aggressions, Atopic dermatitis, Acne | Skin innate defense | Epidermal keratinocytes (NHEK) | Human | Elafin release, beta-defensin 2 release | ELISA / EIA | Basal | |
NHEK, S100A7 expression (basal)| Type of assay | Skin innate defense | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | S100A7 immunolabeling |
| NHEK-0036 | Protection against infections, skin innate immunity, Acne, Wound healing, trauma & agressions, Wound healing, trauma & aggressions, Acne | Skin innate defense | Epidermal keratinocytes (NHEK) | Human | S100A7 immunolabeling | Immunofluorescence | Basal | |
NHEK, innate immunity gene expression (basal)| Type of assay | Skin innate defense | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,..., mQPA-NHEK-IMMUNITY-16 |
| NHEK-0037 | Protection against infections, skin innate immunity, Atopic dermatitis, Acne, Wound healing, trauma & agressions, Wound healing, trauma & aggressions, Atopic dermatitis, Acne | Skin innate defense | Epidermal keratinocytes (NHEK) | Human | mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,..., mQPA-NHEK-IMMUNITY-16 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
NHEK, TLR expression (Pam3CSK4 stimulation)| Type of assay | Gene expression | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | mRNA TLR2 |
| NHEK-0045 | Acne, Acne | Gene expression | Epidermal keratinocytes (NHEK) | Human | mRNA TLR2 | RT-qPCR | Pam3CSK4 | |
NHEK, TLR expression (IFN- gamma stimulation)| Type of assay | Gene expression | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | mRNA TLR2 |
| NHEK-0117 | Acne, Acne | Gene expression | Epidermal keratinocytes (NHEK) | Human | mRNA TLR2 | RT-qPCR | IFN- gamma | |
NHEK, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | 5- alpha reductase activity |
| NHEK-0124 | Hair growth & degeneration, Acne, Acne | Androgen metabolism | Epidermal keratinocytes (NHEK) | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
Sebocytes, proliferation (basal)| Type of assay | Proliferation | | Biological model | SEBO662 sebocyte cell line, BIOalternatives | | Species | Human | | Endpoint | Proliferation |
| SEBO-0001 | Acne, Acne, Sebaceous glands, Oily skin, Dry skin | Proliferation | SEBO662 sebocyte cell line, BIOalternatives | Human | Proliferation | BrdU incorporation (Photometry) + MTT reduction assay (image capture) | Basal | |
Sebocytes, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | SEBO662 sebocyte cell line, BIOalternatives | | Species | Human | | Endpoint | 5- alpha reductase activity |
| SEBO-0003 | Acne, Acne, Sebaceous glands, Oily skin | Androgen metabolism | SEBO662 sebocyte cell line, BIOalternatives | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
3D sebocytes, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | 5- alpha reductase activity |
| SEBO3D-0001 | Acne, Acne, Sebaceous glands, Oily skin | Androgen metabolism | SEBO662 3D model, BIOalternatives | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
3D sebocytes, Ki67 expression (proliferation marker) (basal)| Type of assay | Proliferation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Ki67 immunolabeling & tissu thickness |
| SEBO3D-0004 | Acne, Acne, Sebaceous glands, Oily skin, Dry skin | Proliferation | SEBO662 3D model, BIOalternatives | Human | Ki67 immunolabeling & tissu thickness | Immunohistofluorescence | Basal | |
