Acellular, free radical scavenging| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | DPPH |
| BIOCH-0007 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | DPPH | Photometry | | |
Acellular, free radical scavenging| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | H2O2 measurement (Amplex Red) |
| BIOCH-0008 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | H2O2 measurement (Amplex Red) | Fluorescence | | |
Acellular, free radical scavenging| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | ORAC |
| BIOCH-0009 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | ORAC | Photometry | | |
TBARS formation| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | TBARS production |
| BIOCH-0025 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | TBARS production | Fluorescence | Hemin | |
RHE, sunburn cells (UV stimulation)| Type of assay | DNA damage/apoptosis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Sunburn cell determination |
| EPIBA-0003 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | DNA damage/apoptosis | Reconstructed Epidermis, BIOalternatives RHE | Human | Sunburn cell determination | Histology (HE staining) | UVB | |
RHE, gene expression (UV stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 |
| EPIBA-0004 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 | RT-qPCR, qPCRarray (standard or customized) | UVB | |
RHE, CPD determination (UV stimulation)| Type of assay | DNA damage/apoptosis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | DNA cyclobutane pyrimidine dimers |
| EPIBA-0010 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | DNA damage/apoptosis | Reconstructed Epidermis, BIOalternatives RHE | Human | DNA cyclobutane pyrimidine dimers | Immunohistofluorescence | UVB | |
RHE, stress marker mRNA (basal)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 |
| EPIBA-0066 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
NHEK, gene expression (UV stimulation)| Type of assay | Gene expression | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-NHEK-UVB-32 |
| NHEK-0003 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Epidermal keratinocytes (NHEK) | Human | Selection of genes of interest, mQPA-NHEK-UVB-32 | RT-qPCR, qPCRarray (standard or customized) | UVB | |
NHEK, H2O2 content (UV stimulation)| Type of assay | Free radicals | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | H2O2, DHR probe |
| NHEK-0005 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Free radicals | Epidermal keratinocytes (NHEK) | Human | H2O2, DHR probe | Flow cytometry | UVB | |
NHEK, lipid peroxide content (UV stimulation)| Type of assay | Free radicals | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | Lipid peroxide, C11-Fluor probe |
| NHEK-0006 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Free radicals | Epidermal keratinocytes (NHEK) | Human | Lipid peroxide, C11-Fluor probe | Flow cytometry | UVB | |
NHEK, JNK1/2 activation (H2O2 stimulation)| Type of assay | Signal transduction | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | JNK1/2 phosphorylation |
| NHEK-0109 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Signal transduction | Epidermal keratinocytes (NHEK) | Human | JNK1/2 phosphorylation | Immunofluorescence | H2O2 | |
NHEK, JNK1/2 activation (UV stimulation)| Type of assay | Signal transduction | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | JNK1/2 phosphorylation |
| NHEK-0110 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Signal transduction | Epidermal keratinocytes (NHEK) | Human | JNK1/2 phosphorylation | Immunofluorescence | UVB | |
NHEK, stress marker mRNA (basal)| Type of assay | Gene expression | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 |
| NHEK-0129 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Epidermal keratinocytes (NHEK) | Human | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
Skin maintained ex vivo, sunburn cells (UV stimulation)| Type of assay | DNA damage/apoptosis | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Sunburn cell determination |
| SKIN-0001 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | DNA damage/apoptosis | Skin (ex vivo) | Human | Sunburn cell determination | Histology (HE staining) | UVB | |
Skin maintained ex vivo, CPD determination (UV stimulation)| Type of assay | DNA damage/apoptosis | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | DNA cyclobutane pyrimidine dimers |
| SKIN-0002 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | DNA damage/apoptosis | Skin (ex vivo) | Human | DNA cyclobutane pyrimidine dimers | Immunohistofluorescence | UVB | |
Skin maintained ex vivo, LC, morphology/number (UV stimulation)| Type of assay | Langerhans cells | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | CD1a immunolabeling |
| SKIN-0004 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Langerhans cells | Skin (ex vivo) | Human | CD1a immunolabeling | Immunohistofluorescence | UVB | |
Skin maintained ex vivo, lipid peroxide content (UV stimulation)| Type of assay | Free radicals | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Lipid peroxide quantification |
| SKIN-0005 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Free radicals | Skin (ex vivo) | Human | Lipid peroxide quantification | Photometry | UVB | |
