NHEK, gene expression (poly (I:C) + IL-1 alpha stimulation) Type of assay Gene expression Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint mRNA for early activation markers e.g. TSLP, IFNB1, ..., mQPA-NHEK-AD(1)-16
NHEK-0090 Atopic dermatitis, Atopic dermatitis Gene expression Epidermal keratinocytes (NHEK) Human mRNA for early activation markers e.g. TSLP, IFNB1, ..., mQPA-NHEK-AD(1)-16 RT-qPCR, qPCRarray (standard or customized) Poly(I:C) / IL-1 alpha NHEK, TSLP release (poly (I:C) + IL-1 alpha stimulation) Type of assay Cytokines/chemokines Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint TSLP release
NHEK-0091 Atopic dermatitis, Atopic dermatitis Cytokines/chemokines Epidermal keratinocytes (NHEK) Human TSLP release ELISA / EIA Poly(I:C) / IL-1 alpha NHEK, JNK1/2 activation (H2O2 stimulation) Type of assay Signal transduction Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint JNK1/2 phosphorylation
NHEK-0109 UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) Signal transduction Epidermal keratinocytes (NHEK) Human JNK1/2 phosphorylation Immunofluorescence H2O2 NHEK, JNK1/2 activation (UV stimulation) Type of assay Signal transduction Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint JNK1/2 phosphorylation
NHEK-0110 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) Signal transduction Epidermal keratinocytes (NHEK) Human JNK1/2 phosphorylation Immunofluorescence UVB NHEK, TLR expression (IFN- gamma stimulation) Type of assay Gene expression Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint mRNA TLR2
NHEK-0117 Acne, Acne Gene expression Epidermal keratinocytes (NHEK) Human mRNA TLR2 RT-qPCR IFN- gamma NHEK, testosterone metabolism & 5- alpha reductase activity (basal) Type of assay Androgen metabolism Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint 5- alpha reductase activity
NHEK-0124 Hair growth & degeneration, Acne, Acne Androgen metabolism Epidermal keratinocytes (NHEK) Human 5- alpha reductase activity [14C]-testosterone incorporation Basal NHEK, stress marker mRNA (basal) Type of assay Gene expression Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64
NHEK-0129 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) Gene expression Epidermal keratinocytes (NHEK) Human Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 RT-qPCR, qPCRarray (standard or customized) Basal NHEK, calcium mobilization (basal) Type of assay Signal transduction Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint Calcium mobilization
NHEK-0141 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Signal transduction Epidermal keratinocytes (NHEK) Human Calcium mobilization Time-lapse microscopy Basal NHEK, calcium mobilization (histamine stimulation) Type of assay Signal transduction Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint Calcium mobilization
NHEK-0145 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules, Atopic dermatitis, Atopic dermatitis Signal transduction Epidermal keratinocytes (NHEK) Human Calcium mobilization Time-lapse microscopy Histamine NHEK, calcium mobilization (basal) Type of assay Signal transduction Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint Calcium mobilization
NHEK-0146 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Signal transduction Epidermal keratinocytes (NHEK) Human Calcium mobilization Time-lapse microscopy Basal NHEK, gene expression (PMA stimulation) Type of assay Gene expression Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint Your gene(s) of interest (RT-qPCR), mQPA-INFLAM-TISSUE-64, mQPA-NHEK-64
NHEK-0159 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Gene expression Epidermal keratinocytes (NHEK) Human Your gene(s) of interest (RT-qPCR), mQPA-INFLAM-TISSUE-64, mQPA-NHEK-64 RT-qPCR, qPCRarray (standard or customized) PMA NHEK, gene expression (Poly(I:C) stimulation) Type of assay Gene expression Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint qPCRarray (16 genes)
NHEK-0163 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Gene expression Epidermal keratinocytes (NHEK) Human qPCRarray (16 genes) qPCRarray (standard or customized) Poly(I:C) NHEK, cytokine/chemokine release (poly(I:C) stimulation) Type of assay Cytokines/chemokines Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint IL-6 release, CXCL8/IL-8 release, CCL20 release
NHEK-0164 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Cytokines/chemokines Epidermal keratinocytes (NHEK) Human IL-6 release, CXCL8/IL-8 release, CCL20 release ELISA / EIA Poly(I:C) NHEK, cytokine/chemokine release (IL-1 alpha stimulation) Type of assay Cytokines/chemokines Biological model Epidermal keratinocytes (NHEK) Species Human Endpoint IL-8 release, CCL20 release
NHEK-0167 Psoriasis, Psoriasis Cytokines/chemokines Epidermal keratinocytes (NHEK) Human IL-8 release, CCL20 release ELISA / EIA IL-1 alpha NHEM, stimulation of melanin synthesis (Basal, pro-pigmenting agents) Type of assay Melanin synthesis Biological model Epidermal melanocytes (NHEM) Species Human Endpoint Melanin content
NHEM-0001 Melanogenesis, Pigmentation disorders Melanin synthesis Epidermal melanocytes (NHEM) Human Melanin content Photometry Basal NHEM, inhibition of melanin synthesis (MSH-induced, whitening agents) Type of assay Melanin synthesis Biological model Epidermal melanocytes (NHEM) Species Human Endpoint Melanin content
NHEM-0002 Melanogenesis, Pigmentation disorders Melanin synthesis Epidermal melanocytes (NHEM) Human Melanin content Photometry NDP-MSH NHEM, tyrosinase expression (basal) Type of assay Melanogenic proteins Biological model Epidermal melanocytes (NHEM) Species Human Endpoint Tyrosinase expression
NHEM-0003 Melanogenesis, Pigmentation disorders Melanogenic proteins Epidermal melanocytes (NHEM) Human Tyrosinase expression Immunofluorescence Basal NHEM, TRP-1 expression (basal) Type of assay Melanogenic proteins Biological model Epidermal melanocytes (NHEM) Species Human Endpoint TRP-1 expression
NHEM-0005 Melanogenesis, Pigmentation disorders Melanogenic proteins Epidermal melanocytes (NHEM) Human TRP-1 expression Immunofluorescence Basal NHEM, tyrosinase activity (basal) Type of assay Melanogenic proteins Biological model Epidermal melanocytes (NHEM) Species Human Endpoint Tyrosinase activity
NHEM-0007 Melanogenesis, Pigmentation disorders Melanogenic proteins Epidermal melanocytes (NHEM) Human Tyrosinase activity Photometry Basal NHEM, pigmentation-related gene expression (basal) Type of assay Gene expression Biological model Epidermal melanocytes (NHEM) Species Human Endpoint mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-NHEM-16, mQPA-NHEM-64
NHEM-0009 Melanogenesis, Pigmentation disorders Gene expression Epidermal melanocytes (NHEM) Human mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-NHEM-16, mQPA-NHEM-64 RT-qPCR, qPCRarray (standard or customized) Basal
