3D sebocytes, Ki67 expression (proliferation marker) (basal) Type of assay Proliferation Biological model SEBO662 3D model, BIOalternatives Species Human Endpoint Ki67 immunolabeling & tissu thickness
SEBO3D-0004 Acne, Acne, Sebaceous glands, Oily skin, Dry skin Proliferation SEBO662 3D model, BIOalternatives Human Ki67 immunolabeling & tissu thickness Immunohistofluorescence Basal FTSK, procollagen I expression (basal) Type of assay Protein markers Biological model Full thickness reconstructed skin, BIOalternatives Species Human Endpoint Procollagen I immunolabeling
SKIBA-0017 ECM synthesis, Wound healing, trauma & agressions, Wound healing, trauma & aggressions Protein markers Full thickness reconstructed skin, BIOalternatives Human Procollagen I immunolabeling Immunohistofluorescence or IHC Basal FTSK, fibroblast & ECM-related gene expression (basal) Type of assay Gene expression Biological model Full thickness reconstructed skin, BIOalternatives Species Human Endpoint mRNA: e.g. ECM-related genes, DEJ-related genes..., mQPA-NHDF-16, mQPA-NHDF-32, mQPA-NHDF-64
SKIBA-0024 ECM synthesis, Wound healing, trauma & agressions, Wound healing, trauma & aggressions Gene expression Full thickness reconstructed skin, BIOalternatives Human mRNA: e.g. ECM-related genes, DEJ-related genes..., mQPA-NHDF-16, mQPA-NHDF-32, mQPA-NHDF-64 RT-qPCR, qPCRarray (standard or customized) Basal Skin maintained ex vivo, sunburn cells (UV stimulation) Type of assay DNA damage/apoptosis Biological model Skin (ex vivo) Species Human Endpoint Sunburn cell determination
SKIN-0001 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) DNA damage/apoptosis Skin (ex vivo) Human Sunburn cell determination Histology (HE staining) UVB Skin maintained ex vivo, CPD determination (UV stimulation) Type of assay DNA damage/apoptosis Biological model Skin (ex vivo) Species Human Endpoint DNA cyclobutane pyrimidine dimers
SKIN-0002 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) DNA damage/apoptosis Skin (ex vivo) Human DNA cyclobutane pyrimidine dimers Immunohistofluorescence UVB Skin samples, DC/LC immunolabeling Type of assay In situ immunolabeling Biological model Skin (ex vivo) Species Human Endpoint CD1a immunolabeling, CD207 / Langerhin, CD209/DC-SIGN
SKIN-0003 Dendritic & Langerhans cells, Dendritic & Langerhans cells: inflammation, innate & adaptative immunity In situ immunolabeling Skin (ex vivo) Human CD1a immunolabeling, CD207 / Langerhin, CD209/DC-SIGN Immunohistofluorescence Skin maintained ex vivo, LC, morphology/number (UV stimulation) Type of assay Langerhans cells Biological model Skin (ex vivo) Species Human Endpoint CD1a immunolabeling
SKIN-0004 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) Langerhans cells Skin (ex vivo) Human CD1a immunolabeling Immunohistofluorescence UVB Skin maintained ex vivo, lipid peroxide content (UV stimulation) Type of assay Free radicals Biological model Skin (ex vivo) Species Human Endpoint Lipid peroxide quantification
SKIN-0005 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) Free radicals Skin (ex vivo) Human Lipid peroxide quantification Photometry UVB Skin maintained ex vivo, TRP-1 expression (basal) Type of assay Melanogenic proteins Biological model Skin (ex vivo) Species Human Endpoint TRP-1 immunolabeling
SKIN-0014 Melanogenesis, Pigmentation disorders Melanogenic proteins Skin (ex vivo) Human TRP-1 immunolabeling Immunohistofluorescence Basal Skin maintained ex vivo, Pmel17 expression (basal) Type of assay Melanosomes Biological model Skin (ex vivo) Species Human Endpoint Pmel17 immunolabeling
SKIN-0017 Melanin transfer, Pigmentation disorders Melanosomes Skin (ex vivo) Human Pmel17 immunolabeling Immunohistofluorescence Basal Skin maintained ex vivo, pigmentation (Fontana-Masson staining) Type of assay Melanin synthesis Biological model Skin (ex vivo) Species Human Endpoint Melanin staining
SKIN-0020 Melanogenesis, Skin morphology, Pigmentation disorders Melanin synthesis Skin (ex vivo) Human Melanin staining Histology (Fontana-Masson staining) Basal Skin maintained ex vivo, in situ tyrosinase activity (basal) Type of assay Melanogenic proteins Biological model Skin (ex vivo) Species Human Endpoint Tyrosinase activity
SKIN-0024 Melanogenesis, Pigmentation disorders Melanogenic proteins Skin (ex vivo) Human Tyrosinase activity In situ fluorescence Basal Skin maintained ex vivo, PGE2 release (arachidonic acid stimulation) Type of assay Arachidonic acid metabolites Biological model Skin (ex vivo) Species Human Endpoint PGE2 release
SKIN-0043 Keratinocyte response to small bioactive molecules Arachidonic acid metabolites Skin (ex vivo) Human PGE2 release ELISA / EIA Arachidonic acid Splenocyte proliferation (basal) Type of assay Proliferation and/or migration Biological model Splenocytes Species Mouse Endpoint Proliferation
SPLE-0001 T lymphocytes: immunostimulation, T lymphocytes: immunostimulation, cell-mediated and humoral immunity help Proliferation and/or migration Splenocytes Mouse Proliferation [3H]-thymidine incorporation Basal THP-1, IL-1 beta release (LPS stimulation) Type of assay Inflammatory cytokines Biological model Macrophage-like THP-1 (PMA-differentiated) Species Human Endpoint IL-1 beta release
THP1-0004 Monocytes/macrophages, Monocytes/macrophages: inflammation, innate immunity Inflammatory cytokines Macrophage-like THP-1 (PMA-differentiated) Human IL-1 beta release ELISA / EIA LPS THP-1, caspase 1, enzymatic activity (basal) Type of assay Enzyme activity Biological model THP-1 cell line Species Human Endpoint Caspase-1 activity
THP1-0006 Monocytes/macrophages, Monocytes/macrophages: inflammation, innate immunity Enzyme activity THP-1 cell line Human Caspase-1 activity Fluorescence Basal Thymocytes, caspase 3 (glucocorticoid stimulation) Type of assay Glucocorticoid effect modulation Biological model Thymocytes Species Mouse Endpoint Active caspase 3
THYM-0001 T lymphocytes: immunostimulation, T lymphocytes: immunostimulation, cell-mediated and humoral immunity help Glucocorticoid effect modulation Thymocytes Mouse Active caspase 3 Flow cytometry Dexamethasone Thymocytes, caspase 3 (basal) Type of assay Apoptosis Biological model Thymocytes Species Mouse Endpoint Active caspase 3
THYM-0002 T lymphocytes: anti-inflammatory effects/immunosuppression, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Apoptosis Thymocytes Mouse Active caspase 3 Flow cytometry Basal Psoriatic T cell line, cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Th17 polarity - Cytokines Biological model T cells derived from psoriatic lesions Species Human Endpoint IL-17 release, or other Th17 cytokines
TLPSO-0001 T lymphocytes: anti-inflammatory effects/immunosuppression, Psoriasis, Psoriasis, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Th17 polarity - Cytokines T cells derived from psoriatic lesions Human IL-17 release, or other Th17 cytokines ELISA / EIA Anti-CD3 / Anti-CD28 Psoriatic T cell line, multiplex cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Multiplex cytokines Biological model T cells derived from psoriatic lesions Species Human Endpoint Cytokines, multiplex
TLPSO-0002 T lymphocytes: anti-inflammatory effects/immunosuppression, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Multiplex cytokines T cells derived from psoriatic lesions Human Cytokines, multiplex Flow cytometry (CBA) Anti-CD3 / Anti-CD28
