Blood, particle phagocytosis Type of assay Phagocytosis Biological model Whole Blood Species Human Endpoint Particle phagocytosis
BLOO-0002 Granulocytes, Granulocytes: inflammation & host defense Phagocytosis Whole Blood Human Particle phagocytosis Flow cytometry 37°C Blood, human basophil activation assay (BAT) Type of assay Degranulation Biological model Whole Blood Species Human Endpoint CD63 expression
BLOO-0008 Immuno-inflammation assays using blood/immune cells, Atopic dermatitis, Atopic dermatitis Degranulation Whole Blood Human CD63 expression Flow cytometry Basal Blood, H2O2 content (PMA stimulation) Type of assay Free radicals Biological model Whole Blood Species Human Endpoint H2O2 (cellular), DHR probe
BLOO-0011 Granulocytes, Granulocytes: inflammation & host defense Free radicals Whole Blood Human H2O2 (cellular), DHR probe Flow cytometry PMA CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Th1 polarity - Cytokines Biological model CD4+ T cells Species Human Endpoint IFN- gamma release, TNF- alpha release, or other Th1 cytokines
CD4TL-0001 T lymphocytes: anti-inflammatory effects/immunosuppression, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Th1 polarity - Cytokines CD4+ T cells Human IFN- gamma release, TNF- alpha release, or other Th1 cytokines ELISA / EIA Anti-CD3 / Anti-CD28 CD4+ T cells, multiplex cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Multiplex cytokines Biological model CD4+ T cells Species Human Endpoint Cytokines, multiplex, IL-2, IL-10, IFN- gamma, TNF- alpha, multiplex
CD4TL-0002 T lymphocytes: anti-inflammatory effects/immunosuppression, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Multiplex cytokines CD4+ T cells Human Cytokines, multiplex, IL-2, IL-10, IFN- gamma, TNF- alpha, multiplex Flow cytometry (CBA) Anti-CD3 / Anti-CD28 CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Th2 polarity - Cytokines Biological model CD4+ T cells Species Human Endpoint TNF- alpha release, IL-10 release, or other Th2 cytokines, IL-4 release
CD4TL-0005 T lymphocytes: anti-inflammatory effects/immunosuppression, Atopic dermatitis, Atopic dermatitis, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Th2 polarity - Cytokines CD4+ T cells Human TNF- alpha release, IL-10 release, or other Th2 cytokines, IL-4 release ELISA / EIA Anti-CD3 / Anti-CD28 CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Th17 polarity - Cytokines Biological model CD4+ T cells Species Human Endpoint IL-17 release, or other Th17 cytokines
CD4TL-0007 T lymphocytes: anti-inflammatory effects/immunosuppression, Psoriasis, Psoriasis, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Th17 polarity - Cytokines CD4+ T cells Human IL-17 release, or other Th17 cytokines ELISA / EIA Anti-CD3 / Anti-CD28 CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation) Type of assay Treg polarity - Cytokines Biological model CD4+ T cells Species Human Endpoint IL-10 release, or other Treg cytokines
CD4TL-0009 T lymphocytes: anti-inflammatory effects/immunosuppression, T lymphocytes: anti-inflammatory effects/immunosuppression - cell-mediated and humoral immunity modulation Treg polarity - Cytokines CD4+ T cells Human IL-10 release, or other Treg cytokines ELISA / EIA Anti-CD3 / Anti-CD28 DE, hyaluronic acid synthesis/release (basal) Type of assay GAG synthesis Biological model Dermis-equivalent, normal fibroblasts Species Human Endpoint Hyaluronic acid synthesis / release
DE-0006 ECM synthesis, Wound healing, trauma & agressions, Wound healing, trauma & aggressions GAG synthesis Dermis-equivalent, normal fibroblasts Human Hyaluronic acid synthesis / release ELISA / EIA Basal DE, total GAGs neosynthesis (basal) Type of assay GAG synthesis Biological model Dermis-equivalent, normal fibroblasts Species Human Endpoint Glycosaminoglycans (GAGs, total)
DE-0008 ECM synthesis, Wound healing, trauma & agressions, Wound healing, trauma & aggressions GAG synthesis Dermis-equivalent, normal fibroblasts Human Glycosaminoglycans (GAGs, total) [3H]-glucosamine incorporation Basal DE, fibroblast & ECM-related gene expression (basal) Type of assay Gene expression Biological model Dermis-equivalent, normal fibroblasts Species Human Endpoint mRNA: e.g. ECM-related genes,..., mQPA-NHDF-16, mQPA-NHDF-32, mQPA-NHDF-64
DE-0010 ECM synthesis, Wound healing, trauma & agressions, Wound healing, trauma & aggressions Gene expression Dermis-equivalent, normal fibroblasts Human mRNA: e.g. ECM-related genes,..., mQPA-NHDF-16, mQPA-NHDF-32, mQPA-NHDF-64 RT-qPCR, qPCRarray (standard or customized) Basal DRG network (basal) Type of assay Neurite outgrowth Biological model Dorsal root ganglia (DRG) Species Rat Endpoint beta-III tubulin labeling
DRG-0001 Wound healing, trauma & agressions, Neurodegenerative diseases, Wound healing, trauma & aggressions Neurite outgrowth Dorsal root ganglia (DRG) Rat beta-III tubulin labeling Immunofluorescence Basal HMVEC + NHDF, differentiation (basal, pro-angiogenic agents) Type of assay Differentiation Biological model HMVEC + Fibroblasts, coculture Species Human Endpoint Pseudotube formation
ENDOF-0001 Vascularization, Wound healing, trauma & agressions, Wound healing, trauma & aggressions Differentiation HMVEC + Fibroblasts, coculture Human Pseudotube formation Fluorescence / imaging Basal HMVEC + NHDF, differentiation (VEGF stimulation, anti-angiogenic agents) Type of assay Differentiation Biological model HMVEC + Fibroblasts, coculture Species Human Endpoint Pseudotube formation
ENDOF-0002 Vascularization, Wound healing, trauma & agressions, Wound healing, trauma & aggressions Differentiation HMVEC + Fibroblasts, coculture Human Pseudotube formation Fluorescence / imaging VEGF Epidermis/Dermis, keratinocyte migration (basal) Type of assay Proliferation and/or migration Biological model Normal skin epidermis onto a dermis-equivalent Species Human Endpoint Migration (+ proliferation)
EPI&DE-0001 Wound healing, trauma & agressions, Wound healing, trauma & aggressions, Proliferation, migration, re-epithelialization Proliferation and/or migration Normal skin epidermis onto a dermis-equivalent Human Migration (+ proliferation) Imaging Basal RHE, IL-8 release (PMA stimulation) Type of assay Cytokines/chemokines Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint IL-8 release
EPIBA-0001 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Cytokines/chemokines Reconstructed Epidermis, BIOalternatives RHE Human IL-8 release ELISA / EIA PMA RHE, PGE2 release (PMA stimulation) Type of assay Arachidonic acid metabolites Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint PGE2 release
EPIBA-0002 Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules Arachidonic acid metabolites Reconstructed Epidermis, BIOalternatives RHE Human PGE2 release ELISA / EIA PMA RHE, sunburn cells (UV stimulation) Type of assay DNA damage/apoptosis Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint Sunburn cell determination
EPIBA-0003 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) DNA damage/apoptosis Reconstructed Epidermis, BIOalternatives RHE Human Sunburn cell determination Histology (HE staining) UVB RHE, gene expression (UV stimulation) Type of assay Gene expression Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64
EPIBA-0004 UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) Gene expression Reconstructed Epidermis, BIOalternatives RHE Human Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 RT-qPCR, qPCRarray (standard or customized) UVB RHE, gene expression + histology (OSM stimulation) Type of assay Gene expression Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint mRNA: e.g. S100A7, KRT10 + histology
EPIBA-0005 Psoriasis, Psoriasis Gene expression Reconstructed Epidermis, BIOalternatives RHE Human mRNA: e.g. S100A7, KRT10 + histology RT-qPCR + HE staining OSM
