DPF, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Human Follicle Dermal Papilla Cells(HFDPC) | | Species | Human | | Endpoint | 5- alpha reductase activity |
| DPF-0001 | Hair growth & degeneration | Androgen metabolism | Human Follicle Dermal Papilla Cells(HFDPC) | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
RHE, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | 5- alpha reductase activity |
| EPIBA-0052 | Hair growth & degeneration, Acne, Acne | Androgen metabolism | Reconstructed Epidermis, BIOalternatives RHE | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
Hair follicle growth (basal)| Type of assay | Morphology | | Biological model | Hair follicle | | Species | Human | | Endpoint | Growth measurement for at least 12 days |
| FOLL-0001 | Hair growth & degeneration | Morphology | Hair follicle | Human | Growth measurement for at least 12 days | Imaging | Basal | |
Hair follicle growth & degeneration (basal)| Type of assay | Morphology | | Biological model | Hair follicle | | Species | Human | | Endpoint | Growth measurement & Morphology until apoptosis |
| FOLL-0002 | Hair growth & degeneration | Morphology | Hair follicle | Human | Growth measurement & Morphology until apoptosis | Imaging | Basal | |
Hair follicle, protein expression (basal)| Type of assay | Protein markers | | Biological model | Hair follicle | | Species | Human | | Endpoint | Hair protein markers, e.g. TGK, fibronectin, ... |
| FOLL-0003 | Hair growth & degeneration | Protein markers | Hair follicle | Human | Hair protein markers, e.g. TGK, fibronectin, ... | Immunohistofluorescence | Basal | |
Hair follicle, gene expression (basal)| Type of assay | Gene expression | | Biological model | Hair follicle | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-HAIR |
| FOLL-0004 | Hair growth & degeneration | Gene expression | Hair follicle | Human | Selection of genes of interest, mQPA-HAIR | RT-qPCR, qPCRarray (standard or customized) | Basal | |
Hair follicle, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Hair follicle | | Species | Human | | Endpoint | 5- alpha reductase activity |
| FOLL-0006 | Hair growth & degeneration | Androgen metabolism | Hair follicle | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
Hair follicle, pigmentation-related gene expression (basal)| Type of assay | Gene expression | | Biological model | Hair follicle | | Species | Human | | Endpoint | mRNA: e.g. TYR, TRP1, MITF, MC1R,..., mQPA-HAIR |
| FOLL-0009 | Hair pigmentation, Melanogenesis, Pigmentation disorders | Gene expression | Hair follicle | Human | mRNA: e.g. TYR, TRP1, MITF, MC1R,..., mQPA-HAIR | RT-qPCR, qPCRarray (standard or customized) | Basal | |
Hair follicle, pigmentation (Fontana Masson staining)| Type of assay | Melanin synthesis | | Biological model | Hair follicle | | Species | Human | | Endpoint | Melanin staining |
| FOLL-0011 | Hair pigmentation | Melanin synthesis | Hair follicle | Human | Melanin staining | Histology (Fontana-Masson staining) | Basal | |
NHDF, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Dermal fibroblasts (NHDF) | | Species | Human | | Endpoint | 5- alpha reductase activity |
| NHDF-0050 | Hair growth & degeneration, Acne, Acne | Androgen metabolism | Dermal fibroblasts (NHDF) | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
NHEK, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | 5- alpha reductase activity |
| NHEK-0124 | Hair growth & degeneration, Acne, Acne | Androgen metabolism | Epidermal keratinocytes (NHEK) | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
Sebocytes, proliferation (basal)| Type of assay | Proliferation | | Biological model | SEBO662 sebocyte cell line, BIOalternatives | | Species | Human | | Endpoint | Proliferation |
| SEBO-0001 | Acne, Acne, Sebaceous glands, Oily skin, Dry skin | Proliferation | SEBO662 sebocyte cell line, BIOalternatives | Human | Proliferation | BrdU incorporation (Photometry) + MTT reduction assay (image capture) | Basal | |
Sebocytes, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | SEBO662 sebocyte cell line, BIOalternatives | | Species | Human | | Endpoint | 5- alpha reductase activity |
| SEBO-0003 | Acne, Acne, Sebaceous glands, Oily skin | Androgen metabolism | SEBO662 sebocyte cell line, BIOalternatives | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
3D sebocytes, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | 5- alpha reductase activity |
| SEBO3D-0001 | Acne, Acne, Sebaceous glands, Oily skin | Androgen metabolism | SEBO662 3D model, BIOalternatives | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
3D sebocytes, sebocyte differentiation markers (basal)| Type of assay | Protein markers | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Epithelial membrane antigen (EMA) & cytokeratin 7 immunolabeling |
| SEBO3D-0002 | Sebaceous glands, Dry skin | Protein markers | SEBO662 3D model, BIOalternatives | Human | Epithelial membrane antigen (EMA) & cytokeratin 7 immunolabeling | Immunohistofluorescence | Basal | |
3D sebocytes, sebocyte differentiation markers (basal)| Type of assay | Gene expression | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | mRNA EMA/MUC1, KRT7, PPARG, FLG |
| SEBO3D-0003 | Sebaceous glands, Dry skin | Gene expression | SEBO662 3D model, BIOalternatives | Human | mRNA EMA/MUC1, KRT7, PPARG, FLG | RT-qPCR | Basal | |
3D sebocytes, Ki67 expression (proliferation marker) (basal)| Type of assay | Proliferation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Ki67 immunolabeling & tissu thickness |
| SEBO3D-0004 | Acne, Acne, Sebaceous glands, Oily skin, Dry skin | Proliferation | SEBO662 3D model, BIOalternatives | Human | Ki67 immunolabeling & tissu thickness | Immunohistofluorescence | Basal | |
3D sebocytes, lipid accumulation (basal)| Type of assay | Lipid accumulation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Lipid staining |
| SEBO3D-0005 | Sebaceous glands, Dry skin | Lipid accumulation | SEBO662 3D model, BIOalternatives | Human | Lipid staining | Histology (Oil Red O Staining) | Basal | |
3D sebocytes, lipid accumulation (basal)| Type of assay | Lipid accumulation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Lipid staining |
| SEBO3D-0011 | Sebaceous glands, Dry skin | Lipid accumulation | SEBO662 3D model, BIOalternatives | Human | Lipid staining | Histology (Soudan black staining) | Basal | |
Skin maintained ex vivo, morphology (Oil Red O staining) (basal)| Type of assay | Histology | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Lipid staining |
| SKIN-0041 | Sebaceous glands, Skin morphology | Histology | Skin (ex vivo) | Human | Lipid staining | Histology (Oil Red O Staining) | Basal | |
