Sebocytes, proliferation (basal)| Type of assay | Proliferation | | Biological model | SEBO662 sebocyte cell line, BIOalternatives | | Species | Human | | Endpoint | Proliferation |
| SEBO-0001 | Acne, Acne, Sebaceous glands, Oily skin, Dry skin | Proliferation | SEBO662 sebocyte cell line, BIOalternatives | Human | Proliferation | BrdU incorporation (Photometry) + MTT reduction assay (image capture) | Basal | |
Sebocytes, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | SEBO662 sebocyte cell line, BIOalternatives | | Species | Human | | Endpoint | 5- alpha reductase activity |
| SEBO-0003 | Acne, Acne, Sebaceous glands, Oily skin | Androgen metabolism | SEBO662 sebocyte cell line, BIOalternatives | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
3D sebocytes, testosterone metabolism & 5- alpha reductase activity (basal)| Type of assay | Androgen metabolism | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | 5- alpha reductase activity |
| SEBO3D-0001 | Acne, Acne, Sebaceous glands, Oily skin | Androgen metabolism | SEBO662 3D model, BIOalternatives | Human | 5- alpha reductase activity | [14C]-testosterone incorporation | Basal | |
3D sebocytes, sebocyte differentiation markers (basal)| Type of assay | Protein markers | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Epithelial membrane antigen (EMA) & cytokeratin 7 immunolabeling |
| SEBO3D-0002 | Sebaceous glands, Dry skin | Protein markers | SEBO662 3D model, BIOalternatives | Human | Epithelial membrane antigen (EMA) & cytokeratin 7 immunolabeling | Immunohistofluorescence | Basal | |
3D sebocytes, sebocyte differentiation markers (basal)| Type of assay | Gene expression | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | mRNA EMA/MUC1, KRT7, PPARG, FLG |
| SEBO3D-0003 | Sebaceous glands, Dry skin | Gene expression | SEBO662 3D model, BIOalternatives | Human | mRNA EMA/MUC1, KRT7, PPARG, FLG | RT-qPCR | Basal | |
3D sebocytes, Ki67 expression (proliferation marker) (basal)| Type of assay | Proliferation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Ki67 immunolabeling & tissu thickness |
| SEBO3D-0004 | Acne, Acne, Sebaceous glands, Oily skin, Dry skin | Proliferation | SEBO662 3D model, BIOalternatives | Human | Ki67 immunolabeling & tissu thickness | Immunohistofluorescence | Basal | |
3D sebocytes, lipid accumulation (basal)| Type of assay | Lipid accumulation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Lipid staining |
| SEBO3D-0005 | Sebaceous glands, Dry skin | Lipid accumulation | SEBO662 3D model, BIOalternatives | Human | Lipid staining | Histology (Oil Red O Staining) | Basal | |
3D sebocytes, lipid accumulation (basal)| Type of assay | Lipid accumulation | | Biological model | SEBO662 3D model, BIOalternatives | | Species | Human | | Endpoint | Lipid staining |
| SEBO3D-0011 | Sebaceous glands, Dry skin | Lipid accumulation | SEBO662 3D model, BIOalternatives | Human | Lipid staining | Histology (Soudan black staining) | Basal | |
Skin maintained ex vivo, morphology (Oil Red O staining) (basal)| Type of assay | Histology | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Lipid staining |
| SKIN-0041 | Sebaceous glands, Skin morphology | Histology | Skin (ex vivo) | Human | Lipid staining | Histology (Oil Red O Staining) | Basal | |
Skin maintained ex vivo, morphology (Soudan black staining) (basal)| Type of assay | Histology | | Biological model | Skin (ex vivo) | | Species | Human | | Endpoint | Lipid staining |
| SKIN-0048 | Sebaceous glands, Skin morphology | Histology | Skin (ex vivo) | Human | Lipid staining | Histology (Soudan black staining) | Basal | |
