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You are viewing records 1-20 out of 175 total records on 9 pages
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Plus d'options Reference Physio-pathology Type of assay Biological model Species Endpoint Method Stimulating factor / inducer
B16, stimulation of melanin synthesis (basal, pro-pigmenting agents)

B16, stimulation of melanin synthesis (basal, pro-pigmenting agents)

Type of assayMelanin synthesis
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointMelanin content
B16-0001Melanogenesis, Pigmentation disordersMelanin synthesisB16 melanoma cell lineMouseMelanin contentPhotometryBasal
B16, inhibition of melanin synthesis (MSH-induced, whitening agents)

B16, inhibition of melanin synthesis (MSH-induced, whitening agents)

Type of assayMelanin synthesis
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointMelanin content
B16-0002Melanogenesis, Pigmentation disordersMelanin synthesisB16 melanoma cell lineMouseMelanin contentPhotometryNDP-MSH
B16, tyrosinase expression (basal)

B16, tyrosinase expression (basal)

Type of assayMelanogenic proteins
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointTyrosinase immunolabeling
B16-0010Melanogenesis, Pigmentation disordersMelanogenic proteinsB16 melanoma cell lineMouseTyrosinase immunolabelingImmunofluorescenceBasal
B16, tyrosinase expression (MSH stimulation)

B16, tyrosinase expression (MSH stimulation)

Type of assayMelanogenic proteins
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointTyrosinase immunolabeling
B16-0011Melanogenesis, Pigmentation disordersMelanogenic proteinsB16 melanoma cell lineMouseTyrosinase immunolabelingImmunofluorescenceNDP-MSH
B16, TRP-1 expression (basal)

B16, TRP-1 expression (basal)

Type of assayMelanogenic proteins
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointTRP-1 immunolabeling
B16-0012Melanogenesis, Pigmentation disordersMelanogenic proteinsB16 melanoma cell lineMouseTRP-1 immunolabelingImmunofluorescenceBasal
B16, TRP-1 expression (MSH stimulation)

B16, TRP-1 expression (MSH stimulation)

Type of assayMelanogenic proteins
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointTRP-1 expression
B16-0013Melanogenesis, Pigmentation disordersMelanogenic proteinsB16 melanoma cell lineMouseTRP-1 expressionImmunofluorescenceNDP-MSH
B16, tyrosinase activity (basal)

B16, tyrosinase activity (basal)

Type of assayMelanogenic proteins
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointTyrosinase activity
B16-0014Melanogenesis, Pigmentation disordersMelanogenic proteinsB16 melanoma cell lineMouseTyrosinase activityPhotometryBasal
B16, pigmentation-related gene expression (basal)

B16, pigmentation-related gene expression (basal)

Type of assayGene expression
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointmRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-B16-16, mQPA-B16-64
B16-0016Melanogenesis, Pigmentation disordersGene expressionB16 melanoma cell lineMousemRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-B16-16, mQPA-B16-64RT-qPCR, qPCRarray (standard or customized)Basal
B16, pigmentation-related gene expression (MSH stimulation)

B16, pigmentation-related gene expression (MSH stimulation)

Type of assayGene expression
Biological modelB16 melanoma cell line
SpeciesMouse
EndpointmRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-B16-16, mQPA-B16-64
B16-0018Melanogenesis, Pigmentation disordersGene expressionB16 melanoma cell lineMousemRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,..., mQPA-B16-16, mQPA-B16-64RT-qPCR, qPCRarray (standard or customized)NDP-MSH
Hyaluronidase inhibition (purified enzyme)

Hyaluronidase inhibition (purified enzyme)

Type of assayEnzyme activity
Biological modelAcellular
SpeciesBovine
EndpointHyaluronidase activity
BIOCH-0005Enzyme activity assays, Epidermal matrix, hydration, ECM degradationEnzyme activityAcellularBovineHyaluronidase activityPhotometryBasal
Acellular, free radical scavenging

Acellular, free radical scavenging

Type of assayFree radicals
Biological modelAcellular
Species
EndpointDPPH
BIOCH-0007UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis)Free radicalsAcellularDPPHPhotometry
Acellular, free radical scavenging

Acellular, free radical scavenging

Type of assayFree radicals
Biological modelAcellular
Species
EndpointH2O2 measurement (Amplex Red)
BIOCH-0008UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis)Free radicalsAcellularH2O2 measurement (Amplex Red)Fluorescence
Acellular, free radical scavenging

Acellular, free radical scavenging

Type of assayFree radicals
Biological modelAcellular
Species
EndpointORAC
BIOCH-0009UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis)Free radicalsAcellularORACPhotometry
Tyrosinase inhibition (mushroom enzyme)

Tyrosinase inhibition (mushroom enzyme)

Type of assayTyrosinase inhibition
Biological modelAcellular
Species
EndpointTyrosinase activity
BIOCH-0010Enzyme activity assays, Melanogenesis, Pigmentation disordersTyrosinase inhibitionAcellularTyrosinase activityPhotometry
Tyrosinase inhibition (NHEM extract)

Tyrosinase inhibition (NHEM extract)

Type of assayTyrosinase inhibition
Biological modelAcellular
SpeciesHuman
EndpointTyrosinase activity
BIOCH-0011Enzyme activity assays, Melanogenesis, Pigmentation disordersTyrosinase inhibitionAcellularHumanTyrosinase activityPhotometryBasal
Caspase 3/7, enzymatic activity

Caspase 3/7, enzymatic activity

Type of assayApoptosis
Biological modelAcellular
SpeciesHuman
EndpointCaspase 3/7 activity
BIOCH-0019Enzyme activity assays, Photo-aging (dermis), Photo-aging, UV, solar (dermis)ApoptosisAcellularHumanCaspase 3/7 activityFluorescence
Ceramidase inhibition (enzyme extract)

Ceramidase inhibition (enzyme extract)

Type of assayLipid synthesis
Biological modelAcellular
Species
EndpointCeramidase activity (basic and acid form)
BIOCH-0020Enzyme activity assays, Differentiation, protection, barrier functionLipid synthesisAcellularCeramidase activity (basic and acid form)[14C]-C16-ceramide degradation
TBARS formation

TBARS formation

Type of assayFree radicals
Biological modelAcellular
Species
EndpointTBARS production
BIOCH-0025UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis)Free radicalsAcellularTBARS productionFluorescenceHemin
Non-enzymatic ECM cross-linking (basal)

Non-enzymatic ECM cross-linking (basal)

Type of assayECM glycation
Biological modelAcellular
SpeciesHuman
EndpointECM glycation
BIOCH-0035Intrinsic aging (dermis)ECM glycationAcellularHumanECM glycation[3H]-glucose incorporationBasal
DE, dermis (containing aged NHDF) strength and cohesion (basal)

DE, dermis (containing aged NHDF) strength and cohesion (basal)

Type of assayECM synthesis/degradation
Biological model
SpeciesHuman
EndpointLattices contraction
DE-0014Intrinsic aging (dermis)ECM synthesis/degradationHumanLattices contractionImagingBasal
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