Acellular, free radical scavenging| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | DPPH |
| BIOCH-0007 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | DPPH | Photometry | | |
Acellular, free radical scavenging| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | H2O2 measurement (Amplex Red) |
| BIOCH-0008 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | H2O2 measurement (Amplex Red) | Fluorescence | | |
Acellular, free radical scavenging| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | ORAC |
| BIOCH-0009 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | ORAC | Photometry | | |
TBARS formation| Type of assay | Free radicals | | Biological model | Acellular | | Species | | | Endpoint | TBARS production |
| BIOCH-0025 | UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) | Free radicals | Acellular | | TBARS production | Fluorescence | Hemin | |
RHE, IL-8 release (PMA stimulation)| Type of assay | Cytokines/chemokines | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | IL-8 release |
| EPIBA-0001 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Cytokines/chemokines | Reconstructed Epidermis, BIOalternatives RHE | Human | IL-8 release | ELISA / EIA | PMA | |
RHE, PGE2 release (PMA stimulation)| Type of assay | Arachidonic acid metabolites | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | PGE2 release |
| EPIBA-0002 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Arachidonic acid metabolites | Reconstructed Epidermis, BIOalternatives RHE | Human | PGE2 release | ELISA / EIA | PMA | |
RHE, sunburn cells (UV stimulation)| Type of assay | DNA damage/apoptosis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Sunburn cell determination |
| EPIBA-0003 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | DNA damage/apoptosis | Reconstructed Epidermis, BIOalternatives RHE | Human | Sunburn cell determination | Histology (HE staining) | UVB | |
RHE, gene expression (UV stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 |
| EPIBA-0004 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 | RT-qPCR, qPCRarray (standard or customized) | UVB | |
RHE, CPD determination (UV stimulation)| Type of assay | DNA damage/apoptosis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | DNA cyclobutane pyrimidine dimers |
| EPIBA-0010 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | DNA damage/apoptosis | Reconstructed Epidermis, BIOalternatives RHE | Human | DNA cyclobutane pyrimidine dimers | Immunohistofluorescence | UVB | |
RHE, cadmium cytotoxicity| Type of assay | Cytotoxicity | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | MTT reduction |
| EPIBA-0045 | Pollutant toxicity | Cytotoxicity | Reconstructed Epidermis, BIOalternatives RHE | Human | MTT reduction | Photometry | Cadmium | |
RHE, skin innate immunity markers expression (Basal)| Type of assay | Skin innate defense | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Expression of e.g. S100A7, beta-defensin 2/4, RNAse7 |
| EPIBA-0048 | Protection against infections, skin innate immunity, Wound healing, trauma & agressions, Wound healing, trauma & aggressions | Skin innate defense | Reconstructed Epidermis, BIOalternatives RHE | Human | Expression of e.g. S100A7, beta-defensin 2/4, RNAse7 | Immunohistofluorescence or IHC | Basal | |
RHE, IL-8 release (Basal)| Type of assay | Skin innate defense | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | IL-8 release |
| EPIBA-0049 | Protection against infections, skin innate immunity, Wound healing, trauma & agressions, Wound healing, trauma & aggressions | Skin innate defense | Reconstructed Epidermis, BIOalternatives RHE | Human | IL-8 release | ELISA / EIA | Basal | |
RHE, innate immunity gene expression (Basal)| Type of assay | Skin innate defense | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,..., mQPA-NHEK-IMMUNITY-16 |
| EPIBA-0050 | Protection against infections, skin innate immunity, Wound healing, trauma & agressions, Wound healing, trauma & aggressions | Skin innate defense | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,..., mQPA-NHEK-IMMUNITY-16 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHE, Staphylococcus aureus adhesion (basal)| Type of assay | Bacterial adhesion to skin | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | S. aureus adhesion |
| EPIBA-0060 | Protection against infections, skin innate immunity, Atopic dermatitis, Atopic dermatitis | Bacterial adhesion to skin | Reconstructed Epidermis, BIOalternatives RHE | Human | S. aureus adhesion | Radioactivity | Basal | |
RHE, gene expression (PMA stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Your gene(s) of interest (RT-qPCR), mQPA-INFLAM-TISSUE-64 |
| EPIBA-0061 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Your gene(s) of interest (RT-qPCR), mQPA-INFLAM-TISSUE-64 | RT-qPCR, qPCRarray (standard or customized) | PMA | |
RHE, stress marker mRNA (basal)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 |
| EPIBA-0066 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Selection of genes of interest, mQPA-INFLAM-TISSUE-64, mQPA-OX.STRESS-16, mQPA-OX.STRESS-64 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
NCTC 2544, IL-8 release (PMA stimulation)| Type of assay | Cytokines/chemokines | | Biological model | NCTC 2544 cell line | | Species | Human | | Endpoint | IL-8 release |
| NCTC-0001 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Cytokines/chemokines | NCTC 2544 cell line | Human | IL-8 release | ELISA / EIA | PMA | |
NCTC 2544, PGE2 release (PMA stimulation)| Type of assay | Arachidonic acid metabolites | | Biological model | NCTC 2544 cell line | | Species | Human | | Endpoint | PGE2 release |
| NCTC-0002 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Arachidonic acid metabolites | NCTC 2544 cell line | Human | PGE2 release | ELISA / EIA | PMA | |
NHEK, IL-8 release (PMA stimulation)| Type of assay | Cytokines/chemokines | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | IL-8 release |
| NHEK-0001 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Cytokines/chemokines | Epidermal keratinocytes (NHEK) | Human | IL-8 release | ELISA / EIA | PMA | |
NHEK, gene expression (UV stimulation)| Type of assay | Gene expression | | Biological model | Epidermal keratinocytes (NHEK) | | Species | Human | | Endpoint | Selection of genes of interest, mQPA-NHEK-UVB-32 |
| NHEK-0003 | UV, solar, free radicals, sunburn, UV, solar, sunburn (epidermis) | Gene expression | Epidermal keratinocytes (NHEK) | Human | Selection of genes of interest, mQPA-NHEK-UVB-32 | RT-qPCR, qPCRarray (standard or customized) | UVB | |
