Acellular, free radical scavenging Type of assay Free radicals Biological model Acellular Species Endpoint DPPH
BIOCH-0007 UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) Free radicals Acellular DPPH Photometry Acellular, free radical scavenging Type of assay Free radicals Biological model Acellular Species Endpoint H2O2 measurement (Amplex Red)
BIOCH-0008 UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) Free radicals Acellular H2O2 measurement (Amplex Red) Fluorescence Acellular, free radical scavenging Type of assay Free radicals Biological model Acellular Species Endpoint ORAC
BIOCH-0009 UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) Free radicals Acellular ORAC Photometry Caspase 3/7, enzymatic activity Type of assay Apoptosis Biological model Acellular Species Human Endpoint Caspase 3/7 activity
BIOCH-0019 Enzyme activity assays, Photo-aging (dermis), Photo-aging, UV, solar (dermis) Apoptosis Acellular Human Caspase 3/7 activity Fluorescence TBARS formation Type of assay Free radicals Biological model Acellular Species Endpoint TBARS production
BIOCH-0025 UV, solar, free radicals, sunburn, Miscellaneous free radical assays, UV, solar, sunburn (epidermis) Free radicals Acellular TBARS production Fluorescence Hemin Non-enzymatic ECM cross-linking (basal) Type of assay ECM glycation Biological model Acellular Species Human Endpoint ECM glycation
BIOCH-0035 Intrinsic aging (dermis) ECM glycation Acellular Human ECM glycation [3H]-glucose incorporation Basal DE, dermis (containing aged NHDF) strength and cohesion (basal) Type of assay ECM synthesis/degradation Biological model Species Human Endpoint Lattices contraction
DE-0014 Intrinsic aging (dermis) ECM synthesis/degradation Human Lattices contraction Imaging Basal Jurkat cells, H2O2 content (UV stimulation) Type of assay Free radicals Biological model Jurkat cell line Species Human Endpoint H2O2 (cellular), DHR probe
JURK-0001 Miscellaneous free radical assays, Photo-aging (dermis), Photo-aging, UV, solar (dermis) Free radicals Jurkat cell line Human H2O2 (cellular), DHR probe Flow cytometry UVB Jurkat cells, lipid peroxide content (UV stimulation) Type of assay Free radicals Biological model Jurkat cell line Species Human Endpoint Lipid peroxides (cellular), C11-Fluor
JURK-0003 Miscellaneous free radical assays, Photo-aging (dermis), Photo-aging, UV, solar (dermis), T lymphocytes: immunostimulation, cell-mediated and humoral immunity help Free radicals Jurkat cell line Human Lipid peroxides (cellular), C11-Fluor Flow cytometry UVB NHDF, MMP-1 release (UV stimulation) Type of assay ECM synthesis/degradation Biological model Dermal fibroblasts (NHDF) Species Human Endpoint MMP-1 release
NHDF-0001 ECM degradation, Photo-aging (dermis), Photo-aging, UV, solar (dermis) ECM synthesis/degradation Dermal fibroblasts (NHDF) Human MMP-1 release ELISA / EIA UVA NHDF, gene expression (UV stimulation) Type of assay Gene expression Biological model Dermal fibroblasts (NHDF) Species Human Endpoint Selection of genes of interest, qPCRarray (64 genes)
NHDF-0002 Photo-aging (dermis), Photo-aging, UV, solar (dermis) Gene expression Dermal fibroblasts (NHDF) Human Selection of genes of interest, qPCRarray (64 genes) RT-qPCR, qPCRarray (standard or customized) UVA NHDF, caspase 3/7, enzymatic activity (UV stimulation) Type of assay Apoptosis Biological model Dermal fibroblasts (NHDF) Species Human Endpoint Caspase 3/7 activity
NHDF-0024 Photo-aging (dermis), Photo-aging, UV, solar (dermis) Apoptosis Dermal fibroblasts (NHDF) Human Caspase 3/7 activity Fluorescence UVA NHDF, MMP-1 release (IR stimulation) Type of assay ECM synthesis/degradation Biological model Dermal fibroblasts (NHDF) Species Human Endpoint MMP-1 release
NHDF-0053 Photo-aging (dermis), Photo-aging, UV, solar (dermis) ECM synthesis/degradation Dermal fibroblasts (NHDF) Human MMP-1 release ELISA / EIA IR NHDF, MMP1 gene expression (IR stimulation) Type of assay ECM synthesis/degradation Biological model Dermal fibroblasts (NHDF) Species Human Endpoint mRNA MMP1
NHDF-0084 Photo-aging (dermis), Photo-aging, UV, solar (dermis) ECM synthesis/degradation Dermal fibroblasts (NHDF) Human mRNA MMP1 RT-qPCR IR "Aged" fibroblasts, control of aging, proliferation (basal) Type of assay Proliferation and/or migration Biological model Dermal fibroblasts, replicative aging model (Hayflick) Species Human Endpoint Proliferation
NHDFA1-0001 Intrinsic aging (dermis) Proliferation and/or migration Dermal fibroblasts, replicative aging model (Hayflick) Human Proliferation [3H]-thymidine incorporation Basal "Aged" fibroblasts, control (reversion) of aging, procollagen I & MMP-1 synthesis (basal) Type of assay ECM synthesis/degradation Biological model Dermal fibroblasts, replicative aging model (Hayflick) Species Human Endpoint MMP-1 release, Procollagen I synthesis / release, Procollagen I & MMP-1 synthesis / release
NHDFA1-0002 Intrinsic aging (dermis) ECM synthesis/degradation Dermal fibroblasts, replicative aging model (Hayflick) Human MMP-1 release, Procollagen I synthesis / release, Procollagen I & MMP-1 synthesis / release ELISA / EIA Basal "Aged" fibroblasts, control (reversion) of aging (basal) Type of assay Gene expression Biological model Dermal fibroblasts, replicative aging model (Hayflick) Species Human Endpoint mRNA, e.g. COL1A1, MMP1, ..., mQPA-NHDF AGING-16, mQPA-NHDF AGING-64
NHDFA1-0004 Intrinsic aging (dermis) Gene expression Dermal fibroblasts, replicative aging model (Hayflick) Human mRNA, e.g. COL1A1, MMP1, ..., mQPA-NHDF AGING-16, mQPA-NHDF AGING-64 RT-qPCR, qPCRarray (standard or customized) Basal "Aged" fibroblasts, control (reversion) of aging (basal) Type of assay beta-galactosidase Biological model Dermal fibroblasts, replicative aging model (Hayflick) Species Human Endpoint beta-galactosidase activity
NHDFA1-0005 Intrinsic aging (dermis) beta-galactosidase Dermal fibroblasts, replicative aging model (Hayflick) Human beta-galactosidase activity Microscopy "Aged" fibroblasts, control of aging, migration (basal) Type of assay Proliferation and/or migration Biological model Dermal fibroblasts, replicative aging model (Hayflick) Species Human Endpoint Migration, Migration (+ proliferation)
NHDFA1-0007 Intrinsic aging (dermis) Proliferation and/or migration Dermal fibroblasts, replicative aging model (Hayflick) Human Migration, Migration (+ proliferation) Fluorescence / imaging Basal "Aged" fibroblasts, control (reversion) of aging, total GAGs synthesis (basal) Type of assay ECM synthesis/degradation Biological model Dermal fibroblasts, replicative aging model (Hayflick) Species Human Endpoint Glycosaminoglycans (GAGs, total)
NHDFA1-0009 Intrinsic aging (dermis) ECM synthesis/degradation Dermal fibroblasts, replicative aging model (Hayflick) Human Glycosaminoglycans (GAGs, total) [3H]-glucosamine incorporation Basal
