Molecular biology services

Additionally to our integrated services for pharmacology, our laboratory analyzes your own biological samples using efficient and fully recognized molecular biology methods.

Samples

Cells, tissues,
Cell or tissue extracts,
RNA samples,
Validated cDNA...

Services

Preliminary validation assays using dedicated samples:

Workflow, transfer conditions for optimal results,
Method development,
Method validation (material needed, quantification, range, detection limit).

Study design.

Final sample collection analysis.

Tools & Methods

Sample or tool preparation:

RNA extraction and quality control,
Primer/probe design and validation,
Array plates preparation (ready-to-use qPCR arrays in 384w plates),
Arrays on Nylon, slides or other support.

Transcriptomic analysis:

Single gene expression (RT-qPCR),
Low density (e.g. pathway or cell type) gene expression profiling (qPCRarrays),
Whole genome expression (GenAtlas System, Affymetrix®)

www.affymetrix.com

Mammalian cell line construction/modification:

Gene silencing (Si/ShRNAbased gene silencing),
Protein overexpression.

Information technology

Data and report:
Gene expression data analysis and pathway analysis with Pathway studio software (Ariadne).

In-house Ba®LIMS with fast, reliable and secured information management to generate Data Analysis & Standard Reporting.

Customized reports according to client's specifications also available.

BIOalternatives, 1 bis rue des plantes 86160 GENCAY - France Tel: +33 (0)549.36.11.37
Fax: +33 (0)549.53.10.29