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Plus d'options Reference Physio-pathology Type of assay Biological model Species Endpoint Method Stimulating factor / inducer
3T3-L1, NEFA release (basal)

3T3-L1, NEFA release (basal)

Type of assayTriglyceride hydrolysis
Biological model3T3-L1 preadipocyte cell line (adipocyte-differentiated)
SpeciesMouse
EndpointNon-esterified fatty acid release
3T3L1-0001Lipolysis, LipolysisTriglyceride hydrolysis3T3-L1 preadipocyte cell line (adipocyte-differentiated)MouseNon-esterified fatty acid releasePhotometryBasal
3T3-L1, lipid neosynthesis (basal)

3T3-L1, lipid neosynthesis (basal)

Type of assayLipogenesis
Biological model3T3-L1 preadipocyte cell line (adipocyte-differentiated)
SpeciesMouse
EndpointLipid neosynthesis
3T3L1-0004Lipogenesis, fat storage, Lipogenesis, fat storageLipogenesis3T3-L1 preadipocyte cell line (adipocyte-differentiated)MouseLipid neosynthesis[14C]-acetate incorporationBasal
3T3-L1, lipid neosynthesis (basal)

3T3-L1, lipid neosynthesis (basal)

Type of assayLipogenesis
Biological model3T3-L1 preadipocyte cell line (adipocyte-differentiated)
SpeciesMouse
EndpointLipid neosynthesis
3T3L1-0005Lipogenesis, fat storage, Lipogenesis, fat storageLipogenesis3T3-L1 preadipocyte cell line (adipocyte-differentiated)MouseLipid neosynthesis[3H]-glucose incorporationBasal
3T3-L1, lipid neosynthesis (basal)

3T3-L1, lipid neosynthesis (basal)

Type of assayLipogenesis
Biological model3T3-L1 preadipocyte cell line (adipocyte-differentiated)
SpeciesMouse
EndpointFatty acid uptake
3T3L1-0006Lipogenesis, fat storage, Lipogenesis, fat storageLipogenesis3T3-L1 preadipocyte cell line (adipocyte-differentiated)MouseFatty acid uptake[14C]-oleate incorporationBasal
3T3-L1, active lipoprotein lipase (LPL) production (basal)

3T3-L1, active lipoprotein lipase (LPL) production (basal)

Type of assayLipogenesis
Biological model3T3-L1 preadipocyte cell line (adipocyte-differentiated)
SpeciesMouse
EndpointSynthesis / release of active LPL
3T3L1-0008Lipogenesis, fat storage, Lipogenesis, fat storageLipogenesis3T3-L1 preadipocyte cell line (adipocyte-differentiated)MouseSynthesis / release of active LPLRadioactivityBasal
3T3-L1, differentiation, lipid content/analysis

3T3-L1, differentiation, lipid content/analysis

Type of assayMorphological changes/lipid accumulation
Biological model3T3-L1 preadipocyte cell line
SpeciesMouse
EndpointLipid labeling
3T3L1-0010Adipocyte differentiation, Adipocyte differentiationMorphological changes/lipid accumulation3T3-L1 preadipocyte cell lineMouseLipid labelingFluorescence / imagingDifferentiation mix
Phosphodiesterase inhibition (purified enzyme)

Phosphodiesterase inhibition (purified enzyme)

Type of assaySignal transduction
Biological modelAcellular
SpeciesMouse
EndpointPhosphodiesterase activity
BIOCH-0013Enzyme activity assays, Lipolysis, LipolysisSignal transductionAcellularMousePhosphodiesterase activityRadioactivityBasal
Lipoprotein lipase (LPL) activity

Lipoprotein lipase (LPL) activity

Type of assayEnzyme activity
Biological modelAcellular
SpeciesBovine
EndpointLPL activity
BIOCH-0016Enzyme activity assays, Lipogenesis, fat storage, Lipogenesis, fat storageEnzyme activityAcellularBovineLPL activityRadioactivityBasal
Alpha-amylase inhibition

Alpha-amylase inhibition

Type of assayEnzyme activity
Biological modelAcellular
SpeciesHuman
Endpointalpha-amylase inhibition
BIOCH-0022Enzyme activity assays, Gastroenterology, lipid metabolismEnzyme activityAcellularHumanalpha-amylase inhibitionPhotometryBasal
Pancreatic lipase activity

Pancreatic lipase activity

Type of assayEnzyme activity
Biological modelAcellular
SpeciesPorcine
EndpointPancreatic lipase activity
BIOCH-0026Enzyme activity assays, Lipogenesis, fat storage, Lipogenesis, fat storageEnzyme activityAcellularPorcinePancreatic lipase activityRadioactivityBasal
FABP4 Inhibitor/Ligand screening assay

FABP4 Inhibitor/Ligand screening assay

Type of assayBinding assay
Biological modelAcellular
SpeciesHuman
EndpointFABP4
BIOCH-0030Lipogenesis, fat storageBinding assayAcellularHumanFABP4FluorescenceBasal
Pancreatic lipase activity

Pancreatic lipase activity

Type of assayEnzyme activity
Biological modelAcellular
SpeciesPorcine
EndpointNon-esterified fatty acid quantification
BIOCH-0038Enzyme activity assays, Lipogenesis, fat storage, Lipogenesis, fat storageEnzyme activityAcellularPorcineNon-esterified fatty acid quantificationPhotometryBasal
HMSC differentiation in adipocytes

HMSC differentiation in adipocytes

Type of assayGene expression
Biological modelMesenchymal stem cells (hMSC)
SpeciesHuman
EndpointSelection of genes of interest, qPCRarray (16 genes), qPCRarray (32 genes), qPCRarray (64 genes)
HMSC-0001Adipocyte differentiation, Adipocyte differentiationGene expressionMesenchymal stem cells (hMSC)HumanSelection of genes of interest, qPCRarray (16 genes), qPCRarray (32 genes), qPCRarray (64 genes)RT-qPCR, qPCRarray (standard or customized)Differentiation mix
Adipocytes, NEFA release (basal)

Adipocytes, NEFA release (basal)

Type of assayTriglyceride hydrolysis
Biological modelHypodermal adipocytes (ex vivo)
SpeciesHuman
EndpointNon-esterified fatty acid release
NHA-0001Lipolysis, LipolysisTriglyceride hydrolysisHypodermal adipocytes (ex vivo)HumanNon-esterified fatty acid releasePhotometryBasal
Adipocytes, glycerol release (basal)

Adipocytes, glycerol release (basal)

Type of assayTriglyceride hydrolysis
Biological modelHypodermal adipocytes (ex vivo)
SpeciesHuman
EndpointGlycerol release
NHA-0002Lipolysis, LipolysisTriglyceride hydrolysisHypodermal adipocytes (ex vivo)HumanGlycerol releaseFluorescenceBasal
Adipocytes, cAMP content (basal)

Adipocytes, cAMP content (basal)

Type of assaySignal transduction
Biological modelHypodermal adipocytes (ex vivo)
SpeciesHuman
EndpointcAMP content
NHA-0003Lipolysis, LipolysisSignal transductionHypodermal adipocytes (ex vivo)HumancAMP contentELISA / EIABasal
Adipocytes, lipid neosynthesis (basal)

Adipocytes, lipid neosynthesis (basal)

Type of assayLipogenesis
Biological modelHypodermal adipocytes (ex vivo)
SpeciesHuman
EndpointLipid neosynthesis
NHA-0004Lipogenesis, fat storage, Lipogenesis, fat storageLipogenesisHypodermal adipocytes (ex vivo)HumanLipid neosynthesis[14C]-acetate incorporationBasal
Preadipocytes, NEFA release (basal)

Preadipocytes, NEFA release (basal)

Type of assayTriglyceride hydrolysis
Biological modelDifferentiated preadipocytes
SpeciesHuman
EndpointNon-esterified fatty acid release
PREAD-0001Lipolysis, LipolysisTriglyceride hydrolysisDifferentiated preadipocytesHumanNon-esterified fatty acid releasePhotometryBasal
Preadipocytes, glycerol release (basal)

Preadipocytes, glycerol release (basal)

Type of assayTriglyceride hydrolysis
Biological modelDifferentiated preadipocytes
SpeciesHuman
EndpointGlycerol release
PREAD-0002Lipolysis, LipolysisTriglyceride hydrolysisDifferentiated preadipocytesHumanGlycerol releaseFluorescenceBasal
Preadipocytes, differentiation, lipid content

Preadipocytes, differentiation, lipid content

Type of assayMorphological changes/lipid accumulation
Biological modelPreadipocytes
SpeciesHuman
EndpointLipid labeling
PREAD-0007Adipocyte differentiation, Adipocyte differentiationMorphological changes/lipid accumulationPreadipocytesHumanLipid labelingFluorescence / imagingDifferentiation mix
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