RHE, hyaluronic acid synthesis / release (basal)| Type of assay | GAG synthesis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Hyaluronic acid synthesis / release |
| EPIBA-0028 | Epidermal matrix, hydration | GAG synthesis | Reconstructed Epidermis, BIOalternatives RHE | Human | Hyaluronic acid synthesis / release | ELISA / EIA | Basal | |
RHE, total GAGs neosynthesis (basal)| Type of assay | GAG synthesis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Glycosaminoglycans (GAGs, total) |
| EPIBA-0029 | Epidermal matrix, hydration | GAG synthesis | Reconstructed Epidermis, BIOalternatives RHE | Human | Glycosaminoglycans (GAGs, total) | [3H]-glucosamine incorporation | Basal | |
RHE, sulfated GAGs neosynthesis (basal)| Type of assay | GAG synthesis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Sulfated glycosaminoglycans (sulfated GAGs) |
| EPIBA-0030 | Epidermal matrix, hydration | GAG synthesis | Reconstructed Epidermis, BIOalternatives RHE | Human | Sulfated glycosaminoglycans (sulfated GAGs) | Sulfur-35 (35S) incorporation | Basal | |
RHE, hyaluronic acid expression (basal)| Type of assay | GAG synthesis | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Hyaluronic acid immunolabeling |
| EPIBA-0031 | Epidermal matrix, hydration | GAG synthesis | Reconstructed Epidermis, BIOalternatives RHE | Human | Hyaluronic acid immunolabeling | Immunohistofluorescence | Basal | |
RHE, protein markers (PGs, ECM receptors...) expression (basal)| Type of assay | Protein markers | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Expression of e.g. CD44, alpha2 beta1 integrin... |
| EPIBA-0032 | Epidermal matrix, hydration | Protein markers | Reconstructed Epidermis, BIOalternatives RHE | Human | Expression of e.g. CD44, alpha2 beta1 integrin... | Immunohistofluorescence or IHC | Basal | |
RHE, mRNA for epidermal matrix-related proteins (basal)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. CD44, HAS3, integrins, PGs…, mQPA-NHEK-BARRIER-16 |
| EPIBA-0033 | Epidermal matrix, hydration | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. CD44, HAS3, integrins, PGs…, mQPA-NHEK-BARRIER-16 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHE, dermo-epidermal junction protein expression (basal)| Type of assay | Protein markers | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | other (inquire), Expression of e.g. laminin 5, Collagens IV, VII… |
| EPIBA-0035 | Epidermal cohesion, dermo-epidermal junction | Protein markers | Reconstructed Epidermis, BIOalternatives RHE | Human | other (inquire), Expression of e.g. laminin 5, Collagens IV, VII… | Immunohistofluorescence or IHC | Basal | |
RHE, mRNA for dermo-epidermal junction proteins (basal)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. LAMC2, COL4A1, COL7A1, PGs…, mQPA-NHEK-DEJ-16 |
| EPIBA-0036 | Epidermal cohesion, dermo-epidermal junction | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. LAMC2, COL4A1, COL7A1, PGs…, mQPA-NHEK-DEJ-16 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHE, desmosomal, tight & other cell-cell adhesion proteins (basal)| Type of assay | Protein markers | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Expression of e.g. desmosome proteins, E-cadherin, claudins, occludin … |
| EPIBA-0038 | Epidermal cohesion, cell adhesion & cell junctions | Protein markers | Reconstructed Epidermis, BIOalternatives RHE | Human | Expression of e.g. desmosome proteins, E-cadherin, claudins, occludin … | Immunohistofluorescence or IHC | Basal | |
RHE, mRNA for epidermal adhesion proteins (basal)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. DSG1, DSP, CDH1, CLDN1, OCLN …, mQPA-CELL-JUNCTIONS & COM-32 |
| EPIBA-0039 | Epidermal cohesion, cell adhesion & cell junctions | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. DSG1, DSP, CDH1, CLDN1, OCLN …, mQPA-CELL-JUNCTIONS & COM-32 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHE, gap junction protein expression (basal)| Type of assay | Protein markers | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Expression of e.g. connexin 26, connexin 43… |
| EPIBA-0041 | Intercellular communication | Protein markers | Reconstructed Epidermis, BIOalternatives RHE | Human | Expression of e.g. connexin 26, connexin 43… | Western blot | Basal | |
RHE, mRNA for gap junction proteins (basal)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. GJB2, GJA1..., qPCRarray (32 genes) |
| EPIBA-0042 | Intercellular communication | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. GJB2, GJA1..., qPCRarray (32 genes) | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHE, cadmium cytotoxicity| Type of assay | Cytotoxicity | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | MTT reduction |
| EPIBA-0045 | Pollutant toxicity | Cytotoxicity | Reconstructed Epidermis, BIOalternatives RHE | Human | MTT reduction | Photometry | Cadmium | |
RHE, skin innate immunity markers expression (Basal)| Type of assay | Skin innate defense | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Expression of e.g. S100A7, beta-defensin 2/4, RNAse7 |
| EPIBA-0048 | Protection against infections, skin innate immunity, Wound healing, trauma & agressions, Wound healing, trauma & aggressions | Skin innate defense | Reconstructed Epidermis, BIOalternatives RHE | Human | Expression of e.g. S100A7, beta-defensin 2/4, RNAse7 | Immunohistofluorescence or IHC | Basal | |
RHE, IL-8 release (Basal)| Type of assay | Skin innate defense | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | IL-8 release |
| EPIBA-0049 | Protection against infections, skin innate immunity, Wound healing, trauma & agressions, Wound healing, trauma & aggressions | Skin innate defense | Reconstructed Epidermis, BIOalternatives RHE | Human | IL-8 release | ELISA / EIA | Basal | |
RHE, innate immunity gene expression (Basal)| Type of assay | Skin innate defense | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,..., mQPA-NHEK-IMMUNITY-16 |
| EPIBA-0050 | Protection against infections, skin innate immunity, Wound healing, trauma & agressions, Wound healing, trauma & aggressions | Skin innate defense | Reconstructed Epidermis, BIOalternatives RHE | Human | mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,..., mQPA-NHEK-IMMUNITY-16 | RT-qPCR, qPCRarray (standard or customized) | Basal | |
RHE, glucose consumption (basal)| Type of assay | Metabolism | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Glucose quantification (kinetics) |
| EPIBA-0056 | Keratinocyte viability & metabolism | Metabolism | Reconstructed Epidermis, BIOalternatives RHE | Human | Glucose quantification (kinetics) | Photometry | Basal | |
RHE, epidermal morphology (Hematoxylin-Eosin staining) (basal)| Type of assay | Histology | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Morphology |
| EPIBA-0059 | Keratinocyte viability & metabolism | Histology | Reconstructed Epidermis, BIOalternatives RHE | Human | Morphology | Histology (HE staining) | Basal | |
RHE, Staphylococcus aureus adhesion (basal)| Type of assay | Bacterial adhesion to skin | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | S. aureus adhesion |
| EPIBA-0060 | Protection against infections, skin innate immunity, Atopic dermatitis, Atopic dermatitis | Bacterial adhesion to skin | Reconstructed Epidermis, BIOalternatives RHE | Human | S. aureus adhesion | Radioactivity | Basal | |
RHE, gene expression (PMA stimulation)| Type of assay | Gene expression | | Biological model | Reconstructed Epidermis, BIOalternatives RHE | | Species | Human | | Endpoint | Your gene(s) of interest (RT-qPCR), mQPA-INFLAM-TISSUE-64 |
| EPIBA-0061 | Keratinocyte inflammatory response to small bioactive molecules, Keratinocyte response to small bioactive molecules | Gene expression | Reconstructed Epidermis, BIOalternatives RHE | Human | Your gene(s) of interest (RT-qPCR), mQPA-INFLAM-TISSUE-64 | RT-qPCR, qPCRarray (standard or customized) | PMA | |
