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Plus d'options Reference Physio-pathology Type of assay Biological model Species Endpoint Method Stimulating factor / inducer
DE, dermis (containing aged NHDF) strength and cohesion (basal)

DE, dermis (containing aged NHDF) strength and cohesion (basal)

Type of assayECM synthesis/degradation
Biological model
SpeciesHuman
EndpointLattices contraction
DE-0014Intrinsic aging (dermis)ECM synthesis/degradationHumanLattices contractionImagingBasal
DPF, testosterone metabolism & 5- alpha reductase activity (basal)

DPF, testosterone metabolism & 5- alpha reductase activity (basal)

Type of assayAndrogen metabolism
Biological modelHuman Follicle Dermal Papilla Cells(HFDPC)
SpeciesHuman
Endpoint5- alpha reductase activity
DPF-0001Hair growth & degenerationAndrogen metabolismHuman Follicle Dermal Papilla Cells(HFDPC)Human5- alpha reductase activity[14C]-testosterone incorporationBasal
Epidermis/Dermis, keratinocyte migration (basal)

Epidermis/Dermis, keratinocyte migration (basal)

Type of assayProliferation and/or migration
Biological modelNormal skin epidermis onto a dermis-equivalent
SpeciesHuman
EndpointMigration (+ proliferation)
EPI&DE-0001Wound healing, trauma & agressions, Wound healing, trauma & aggressions, Proliferation, migration, re-epithelializationProliferation and/or migrationNormal skin epidermis onto a dermis-equivalentHumanMigration (+ proliferation)ImagingBasal
RHE, sirtuin 1 expression (basal)

RHE, sirtuin 1 expression (basal)

Type of assayMetabolism
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointSirtuin 1 immunolabeling
EPIBA-0012Keratinocyte viability & metabolismMetabolismReconstructed Epidermis, BIOalternatives RHEHumanSirtuin 1 immunolabelingImmunohistofluorescenceBasal
RHE, mitochondrial dehydrogenase activity (basal)

RHE, mitochondrial dehydrogenase activity (basal)

Type of assayMetabolism
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointMTT reduction
EPIBA-0016Keratinocyte viability & metabolismMetabolismReconstructed Epidermis, BIOalternatives RHEHumanMTT reductionPhotometryBasal
RHE, ATP content (basal)

RHE, ATP content (basal)

Type of assayMetabolism
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointATP
EPIBA-0017Keratinocyte viability & metabolismMetabolismReconstructed Epidermis, BIOalternatives RHEHumanATPLuminescenceBasal
RHE, Ki67 expression (proliferation marker) (basal)

RHE, Ki67 expression (proliferation marker) (basal)

Type of assayProtein markers
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointKi67
EPIBA-0018Proliferation, migration, re-epithelializationProtein markersReconstructed Epidermis, BIOalternatives RHEHumanKi67Immunohistofluorescence or IHCBasal
RHE, [14C]-caffein diffusion (basal)

RHE, [14C]-caffein diffusion (basal)

Type of assayBarrier function
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
Endpoint[14C]-caffein diffusion (long protocol; kinetic)
EPIBA-0019Differentiation, protection, barrier functionBarrier functionReconstructed Epidermis, BIOalternatives RHEHuman[14C]-caffein diffusion (long protocol; kinetic)RadioactivityBasal
RHE, TGK activity in epidermis (basal)

RHE, TGK activity in epidermis (basal)

Type of assayProtein markers
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointTransglutaminase 1 activity
EPIBA-0020Differentiation, protection, barrier functionProtein markersReconstructed Epidermis, BIOalternatives RHEHumanTransglutaminase 1 activityRadiobiochemicalBasal
RHE, differentiation marker expression (basal)

RHE, differentiation marker expression (basal)

Type of assayProtein markers
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointExpression of e.g. filaggrin, TGK, cytokeratin 10, loricrin...
EPIBA-0021Differentiation, protection, barrier functionProtein markersReconstructed Epidermis, BIOalternatives RHEHumanExpression of e.g. filaggrin, TGK, cytokeratin 10, loricrin...Immunohistofluorescence or IHCBasal
RHE, mRNA for differentiation markers (basal)

RHE, mRNA for differentiation markers (basal)

Type of assayGene expression
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointmRNA: e.g. FLG, TGM1, KRT10, LOR…, mQPA-NHEK-16, mQPA-NHEK-32, mQPA-NHEK-64
EPIBA-0022Differentiation, protection, barrier functionGene expressionReconstructed Epidermis, BIOalternatives RHEHumanmRNA: e.g. FLG, TGM1, KRT10, LOR…, mQPA-NHEK-16, mQPA-NHEK-32, mQPA-NHEK-64RT-qPCR, qPCRarray (standard or customized)Basal
RHE, total lipid & ceramide neosynthesis (basal)

RHE, total lipid & ceramide neosynthesis (basal)

Type of assayLipid synthesis
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointTotal lipid & ceramide neosynthesis
EPIBA-0024Differentiation, protection, barrier functionLipid synthesisReconstructed Epidermis, BIOalternatives RHEHumanTotal lipid & ceramide neosynthesis[14C]-acetate incorporation in polar lipids + TLCBasal
RHE, total lipids & ceramide neosynthesis (Vitamin C stimulation)

RHE, total lipids & ceramide neosynthesis (Vitamin C stimulation)

Type of assayLipid synthesis
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointTotal lipid & ceramide neosynthesis
EPIBA-0025Differentiation, protection, barrier functionLipid synthesisReconstructed Epidermis, BIOalternatives RHEHumanTotal lipid & ceramide neosynthesis[14C]-acetate incorporation in polar lipids + TLCVitamin C
RHE, hyaluronic acid synthesis / release (basal)

RHE, hyaluronic acid synthesis / release (basal)

Type of assayGAG synthesis
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointHyaluronic acid synthesis / release
EPIBA-0028Epidermal matrix, hydrationGAG synthesisReconstructed Epidermis, BIOalternatives RHEHumanHyaluronic acid synthesis / releaseELISA / EIABasal
RHE, total GAGs neosynthesis (basal)

RHE, total GAGs neosynthesis (basal)

Type of assayGAG synthesis
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointGlycosaminoglycans (GAGs, total)
EPIBA-0029Epidermal matrix, hydrationGAG synthesisReconstructed Epidermis, BIOalternatives RHEHumanGlycosaminoglycans (GAGs, total)[3H]-glucosamine incorporationBasal
RHE, sulfated GAGs neosynthesis (basal)

RHE, sulfated GAGs neosynthesis (basal)

Type of assayGAG synthesis
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointSulfated glycosaminoglycans (sulfated GAGs)
EPIBA-0030Epidermal matrix, hydrationGAG synthesisReconstructed Epidermis, BIOalternatives RHEHumanSulfated glycosaminoglycans (sulfated GAGs)Sulfur-35 (35S) incorporationBasal
RHE, hyaluronic acid expression (basal)

RHE, hyaluronic acid expression (basal)

Type of assayGAG synthesis
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointHyaluronic acid immunolabeling
EPIBA-0031Epidermal matrix, hydrationGAG synthesisReconstructed Epidermis, BIOalternatives RHEHumanHyaluronic acid immunolabelingImmunohistofluorescenceBasal
RHE, protein markers (PGs, ECM receptors...) expression (basal)

RHE, protein markers (PGs, ECM receptors...) expression (basal)

Type of assayProtein markers
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointExpression of e.g. CD44, alpha2 beta1 integrin...
EPIBA-0032Epidermal matrix, hydrationProtein markersReconstructed Epidermis, BIOalternatives RHEHumanExpression of e.g. CD44, alpha2 beta1 integrin...Immunohistofluorescence or IHCBasal
RHE, mRNA for epidermal matrix-related proteins (basal)

RHE, mRNA for epidermal matrix-related proteins (basal)

Type of assayGene expression
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
EndpointmRNA: e.g. CD44, HAS3, integrins, PGs…, mQPA-NHEK-BARRIER-16
EPIBA-0033Epidermal matrix, hydrationGene expressionReconstructed Epidermis, BIOalternatives RHEHumanmRNA: e.g. CD44, HAS3, integrins, PGs…, mQPA-NHEK-BARRIER-16RT-qPCR, qPCRarray (standard or customized)Basal
RHE, dermo-epidermal junction protein expression (basal)

RHE, dermo-epidermal junction protein expression (basal)

Type of assayProtein markers
Biological modelReconstructed Epidermis, BIOalternatives RHE
SpeciesHuman
Endpointother (inquire), Expression of e.g. laminin 5, Collagens IV, VII…
EPIBA-0035Epidermal cohesion, dermo-epidermal junctionProtein markersReconstructed Epidermis, BIOalternatives RHEHumanother (inquire), Expression of e.g. laminin 5, Collagens IV, VII…Immunohistofluorescence or IHCBasal
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