RHE, mRNA for dermo-epidermal junction proteins (basal) Type of assay Gene expression Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint mRNA: e.g. LAMC2, COL4A1, COL7A1, PGs…, mQPA-NHEK-DEJ-16
EPIBA-0036 Epidermal cohesion, dermo-epidermal junction Gene expression Reconstructed Epidermis, BIOalternatives RHE Human mRNA: e.g. LAMC2, COL4A1, COL7A1, PGs…, mQPA-NHEK-DEJ-16 RT-qPCR, qPCRarray (standard or customized) Basal RHE, desmosomal, tight & other cell-cell adhesion proteins (basal) Type of assay Protein markers Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint Expression of e.g. desmosome proteins, E-cadherin, claudins, occludin …
EPIBA-0038 Epidermal cohesion, cell adhesion & cell junctions Protein markers Reconstructed Epidermis, BIOalternatives RHE Human Expression of e.g. desmosome proteins, E-cadherin, claudins, occludin … Immunohistofluorescence or IHC Basal RHE, mRNA for epidermal adhesion proteins (basal) Type of assay Gene expression Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint mRNA: e.g. DSG1, DSP, CDH1, CLDN1, OCLN …, mQPA-CELL-JUNCTIONS & COM-32
EPIBA-0039 Epidermal cohesion, cell adhesion & cell junctions Gene expression Reconstructed Epidermis, BIOalternatives RHE Human mRNA: e.g. DSG1, DSP, CDH1, CLDN1, OCLN …, mQPA-CELL-JUNCTIONS & COM-32 RT-qPCR, qPCRarray (standard or customized) Basal RHE, gap junction protein expression (basal) Type of assay Protein markers Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint Expression of e.g. connexin 26, connexin 43…
EPIBA-0041 Intercellular communication Protein markers Reconstructed Epidermis, BIOalternatives RHE Human Expression of e.g. connexin 26, connexin 43… Western blot Basal RHE, mRNA for gap junction proteins (basal) Type of assay Gene expression Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint mRNA: e.g. GJB2, GJA1..., qPCRarray (32 genes)
EPIBA-0042 Intercellular communication Gene expression Reconstructed Epidermis, BIOalternatives RHE Human mRNA: e.g. GJB2, GJA1..., qPCRarray (32 genes) RT-qPCR, qPCRarray (standard or customized) Basal RHE, testosterone metabolism & 5- alpha reductase activity (basal) Type of assay Androgen metabolism Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint 5- alpha reductase activity
EPIBA-0052 Hair growth & degeneration, Acne, Acne Androgen metabolism Reconstructed Epidermis, BIOalternatives RHE Human 5- alpha reductase activity [14C]-testosterone incorporation Basal RHE, glucose consumption (basal) Type of assay Metabolism Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint Glucose quantification (kinetics)
EPIBA-0056 Keratinocyte viability & metabolism Metabolism Reconstructed Epidermis, BIOalternatives RHE Human Glucose quantification (kinetics) Photometry Basal RHE, epidermal morphology (Hematoxylin-Eosin staining) (basal) Type of assay Histology Biological model Reconstructed Epidermis, BIOalternatives RHE Species Human Endpoint Morphology
EPIBA-0059 Keratinocyte viability & metabolism Histology Reconstructed Epidermis, BIOalternatives RHE Human Morphology Histology (HE staining) Basal Hair follicle growth (basal) Type of assay Morphology Biological model Hair follicle Species Human Endpoint Growth measurement for at least 12 days
FOLL-0001 Hair growth & degeneration Morphology Hair follicle Human Growth measurement for at least 12 days Imaging Basal Hair follicle growth & degeneration (basal) Type of assay Morphology Biological model Hair follicle Species Human Endpoint Growth measurement & Morphology until apoptosis
FOLL-0002 Hair growth & degeneration Morphology Hair follicle Human Growth measurement & Morphology until apoptosis Imaging Basal Hair follicle, protein expression (basal) Type of assay Protein markers Biological model Hair follicle Species Human Endpoint Hair protein markers, e.g. TGK, fibronectin, ...
FOLL-0003 Hair growth & degeneration Protein markers Hair follicle Human Hair protein markers, e.g. TGK, fibronectin, ... Immunohistofluorescence Basal Hair follicle, gene expression (basal) Type of assay Gene expression Biological model Hair follicle Species Human Endpoint Selection of genes of interest, mQPA-HAIR
FOLL-0004 Hair growth & degeneration Gene expression Hair follicle Human Selection of genes of interest, mQPA-HAIR RT-qPCR, qPCRarray (standard or customized) Basal Hair follicle, testosterone metabolism & 5- alpha reductase activity (basal) Type of assay Androgen metabolism Biological model Hair follicle Species Human Endpoint 5- alpha reductase activity
FOLL-0006 Hair growth & degeneration Androgen metabolism Hair follicle Human 5- alpha reductase activity [14C]-testosterone incorporation Basal Hair follicle, pigmentation-related gene expression (basal) Type of assay Gene expression Biological model Hair follicle Species Human Endpoint mRNA: e.g. TYR, TRP1, MITF, MC1R,..., mQPA-HAIR
FOLL-0009 Hair pigmentation, Melanogenesis, Pigmentation disorders Gene expression Hair follicle Human mRNA: e.g. TYR, TRP1, MITF, MC1R,..., mQPA-HAIR RT-qPCR, qPCRarray (standard or customized) Basal Hair follicle, pigmentation (Fontana Masson staining) Type of assay Melanin synthesis Biological model Hair follicle Species Human Endpoint Melanin staining
FOLL-0011 Hair pigmentation Melanin synthesis Hair follicle Human Melanin staining Histology (Fontana-Masson staining) Basal Jurkat cells, H2O2 content (UV stimulation) Type of assay Free radicals Biological model Jurkat cell line Species Human Endpoint H2O2 (cellular), DHR probe
JURK-0001 Miscellaneous free radical assays, Photo-aging (dermis), Photo-aging, UV, solar (dermis) Free radicals Jurkat cell line Human H2O2 (cellular), DHR probe Flow cytometry UVB Jurkat cells, lipid peroxide content (UV stimulation) Type of assay Free radicals Biological model Jurkat cell line Species Human Endpoint Lipid peroxides (cellular), C11-Fluor
JURK-0003 Miscellaneous free radical assays, Photo-aging (dermis), Photo-aging, UV, solar (dermis), T lymphocytes: immunostimulation, cell-mediated and humoral immunity help Free radicals Jurkat cell line Human Lipid peroxides (cellular), C11-Fluor Flow cytometry UVB NHEK + NHEM, stimulation of melanin synthesis (Basal, pro-pigmenting agents) Type of assay Melanin synthesis Biological model Keratinocytes + Melanocytes, coculture Species Human Endpoint Melanin content
KM-0001 Melanogenesis, Pigmentation disorders Melanin synthesis Keratinocytes + Melanocytes, coculture Human Melanin content Photometry Basal NHEK + NHEM, tyrosinase expression (basal) Type of assay Melanogenic proteins Biological model Keratinocytes + Melanocytes, coculture Species Human Endpoint Tyrosinase immunolabeling
KM-0003 Melanogenesis, Pigmentation disorders Melanogenic proteins Keratinocytes + Melanocytes, coculture Human Tyrosinase immunolabeling Immunofluorescence Basal NHEK + NHEM, TRP-1 expression (basal) Type of assay Melanogenic proteins Biological model Keratinocytes + Melanocytes, coculture Species Human Endpoint TRP-1 immunolabeling
KM-0005 Melanogenesis, Pigmentation disorders Melanogenic proteins Keratinocytes + Melanocytes, coculture Human TRP-1 immunolabeling Immunofluorescence Basal
