Granulocytes: inflammation & host defense
BLOO-0001 : Blood, CD11b/CD62L expression (TNF- alpha stimulation)
- Cell Model :
Whole blood
- Parameter/biomarker/endpoint :
CD11b (increase) / CD62L (decrease) expression
- Method :
Flow cytometry
- Stimulating factor/inducer :
TNF- alpha
Control
TNF-alpha stimulated
BLOO-0002 : Blood, particle phagocytosis
- Cell Model :
Whole blood
- Parameter/biomarker/endpoint :
Particle phagocytosis
- Method :
Flow cytometry
- Stimulating factor/inducer :
37°C
Whole blood cells
PMN-0001 : PMN, CD11b/CD62L expression (TNF- alpha stimulation)
- Cell Model :
Granulocytes
- Parameter/biomarker/endpoint :
CD11b (increase) / CD62L (decrease) expression
- Method :
Flow cytometry
- Stimulating factor/inducer :
TNF- alpha
CD11b+ granulocytes
CD62L+ granulocytes
PMN-0002 : PMN, cytokine release (TNF- alpha stimulation)
- Cell Model :
Granulocytes
- Parameter/biomarker/endpoint :
IL-1 beta release
- Method :
ELISA
- Stimulating factor/inducer :
TNF- alpha
- Reference compound :
Dexamethasone
IL-8
PMN-0003 : PMN, particle phagocytosis
- Cell Model :
Granulocytes
- Parameter/biomarker/endpoint :
Particle phagocytosis
- Method :
Flow cytometry
- Stimulating factor/inducer :
37°C
Control
Temperature stimulated
PMN-0005 : PMN, H2O2 content (PMA stimulation)
- Cell Model :
Granulocytes
- Parameter/biomarker/endpoint :
H2O2 (cellular), DHR probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
PMA
- Reference compound :
BHA
Hydrogen peroxide content
PMN-0007 : PMN, O2- content (PMA stimulation)
- Cell Model :
Granulocytes
- Parameter/biomarker/endpoint :
O2- (cellular), Hydroxyethidine probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
PMA
- Reference compound :
BHA
Superoxide anion radical content
