T lymphocytes: immunostimulation, cell-mediated and humoral immunity help
PBMC-0003 : PBMC, IFN- gamma release (basal)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
IFN- gamma release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
PHA or anti-CD3/anti-CD28
IFN-gamma
PBMC-0004 : PBMC, IFN- gamma release (anti-CD3 stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
IFN- gamma release
- Method :
ELISA
- Stimulating factor/inducer :
Anti-CD3
- Reference compound :
Anti-CD28
IFN-gamma
SPLE-0001 : Splenocyte proliferation (basal)
- Cell Model :
Splenocytes
- Parameter/biomarker/endpoint :
Proliferation
- Method :
[3H]-thymidine incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
PHA
Splenocyte proliferation
THYM-0001 : Thymocytes, caspase 3 (glucocorticoid stimulation)
- Cell Model :
Thymocytes
- Parameter/biomarker/endpoint :
Active caspase 3
- Method :
Flow cytometry
- Stimulating factor/inducer :
Dexamethasone
- Reference compound :
RU486
Active caspase 3
