Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction.

Exp Dermatol. 2008 Jun 4. Epub ahead of print

Saintigny G, Bernard FX, Juchaux F, Pedretti N, Mahé C.
Chanel Parfum Beauté, Neuilly/Seine, France, BIOalternatives, Gençay, France .

As revealed by immunohistochemistry and RT-QPCR, the focal adhesion protein tensin1 is expressed in cultured human dermal fibroblasts and reduced by 60% after transfection with tensin1 siRNA. Tensin1 silenced fibroblast exhibited a strongly reduced capacity to contract collagen gels. Aged fibroblasts, generated with the Hayflick replicative senescence model, exhibit as siRNA silences fibroblasts, a reduced tensin1 expression and an impaired gel contraction capacity. Based on these results, we speculate that in human dermal fibroblasts, tensin1 plays an important role in cell-matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing.

PMID: 18537817 PubMed - as supplied by publisher