FIELDS AND METHODS
BIOalternatives thoroughly uses up-to-date in vitro technologies. Our platform is based on the cellular and molecular pharmacology expertise.
The company proposes access to:
Imaging technologies,
High Content Cellular Screening,
Transcriptomics.
PATHWAYS & TARGETS
Cellular and Molecular Toxicology
- viability
- cytotoxicity profiling
- apoptosis
- phototoxicity...
Differentiation
- migration
- adhesion
- proliferation
- morphology...
Metabolism
- energy
- protein
- lipid...
Extracellular Matrix
- synthesis
- organization
- degradation
Communication
- growth factors
- chemokines
- cytokines,
- neurotransmitters...
Signal Transduction
- Ca2+ mobilization
- cAMP...
Stress
- reactive oxygen species
- intoxication...
TOOLS & METHODS
Cell and tissue culture
- isolation
- typing
- histology...
Bioimaging
- miscroscopy (light, confocal, electron)
- high resolution fixed and living cell analysis
Immunoassays
- ELISA
- flow cytometry
- western blot
- immunochemistry
- immunofluorescence
Molecular Biology
- microarrays
- quantitative RT-PCR
- cell transfection
- siRNA
Biochemistry
- absorbance
- fluorescence,
- chemiluminescence
- radioactivity
