Restricted access
BIOalternatives : State-of-the-art laboratory
InCell Analyzer 1000
High resolution system to visualize the intracellular movements and to study living cells in culture or fixed cells.
BIOalternatives has recently acquired the IN Cell Analyzer 1000, a fully automated, rapid image acquisition and analysis system for cell-based assays. IN Cell Analyzer provides qualitative and quantitative data from high quality microscopy imaging.
Example of assays :
- Proteomics, expression/localization
- Receptor activation
- Signal transduction
- TF nuclear translocation
- Viability, proliferation, apoptosis
- Cell cycle
- Morphology
- Organelle and trafficking studies…
Technical specifications :
- High-resolution CCD camera
- Nikon inverted microscope
- Autofocus laser system
- Motorized stage with fast and high precision well-to-well motion
- Screening formats (96 - 384 well microplates)
- Fluidics & injection for compound dispensing
- Temperature-regulation for living cell-based assays
The analysis software of IN Cell Analyzer allows :
1/ image segmentation : precise fluorescence label location combined to nuclei counting.
Example : determination of the fluorescence intensity per cell, the number of labelled cells (positive)...
2/ cell classification : detection of cells exhibiting a specific phenotype.
Example: identification of « psoriatic » cells from normal cells.
Today BIOalternatives has already validated assays for
- Neurite network quantification
- Neurofilament labeling
- Intracellular calcium mobilization
- Melanocyte dendricity (TRP-1 labeling)
- Keratinocyte differentiation related protein (TGK, FIL, LOR, SPTLC1...)
- Extracellular matrix protein (COLI, COLIII, COLV, FIL...)
- Mitochondrial activity (depolarization)
- Cell counting (hoechst staining)
- Cell death (propidium iodide staining)
An extended range of assays is currently in development.
For more details, please contact us
Neuron network
Green: neurons labeled with anti-neurofilament antibody.
Red: astrocytes labeled with GFAP antibody.
Blue: nuclei stained with Hoechst dye.