Real time PCR (quantitative PCR, qPCR) is the most sensitive and specific method for gene expression analysis, when coupled to mRNA reverse-transcription (RT-qPCR).

Using new automation/miniaturization processes, we now offer solutions for qPCR-based mRNA expression analysis in 384-well plate formats.

In this system each 384 plate contains 8 to 192 cDNAs in duplicate, coming from:

• standard BIOalternatives models
• samples generated by BIOalternatives from your compounds or tested conditions
• cell or tissue, RNA or cDNA samples from your lab

cDNA-qPCR array "Skin models" is designed to provide information regarding the expression profile of a specific gene in a set of 62 skin-related models available at BIOalternatives.
Models are either basal or stressed, more or less differentiated, aged, etc…

• keratinocytes
• adipocytes
• fibroblasts
• mesenchymal cells
• melanocytes
• endothelial cells
• hair
• immune cells...

For example, expression of MMP1 in the 62 samples of the qPCR array “Ba skin model-1”

Additionally, the expression of the target gene is compared to the expression of 3 housekeeping genes and to their average: 5 results provided for each analyzed gene.

1. Send us the GenBank number of your selected genes
2. Receive a full report in Excel format (list of models, raw data, final values, figures) in around 1 week.

Prices :

• 1st gene : 650 € (including 3 housekeeping)
• additional gene : 500 €

Custom cDNA-qPCR arrays and Primer-qPCR arrays are also available now, for more information : contact us

Soon more examples of qPCRarrays...