Adipocyte differentiation
3T3L1-0010 : 3T3-L1, differentiation, lipid content/analysis
- Cell Model :
3T3-L1 preadipocyte cell line
- Parameter/biomarker/endpoint :
Lipid labeling
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
Control
TNF-alpha
3T3L1-0009 : 3T3-L1, differentiation, morphological changes
- Cell Model :
3T3-L1 preadipocyte cell line
- Parameter/biomarker/endpoint :
Morphological changes
- Method :
Microscopy
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
Differentiated
Non-differentiated
HMSC-0001 : HMSC differentiation in adipocytes
- Cell Model :
Mesenchymal stem cells
- Parameter/biomarker/endpoint :
Selection of genes of interest
- Method :
RT-qPCR
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
TNF-alpha
PREAD-0009 : Preadipocytes, adipogenic differentiation markers (differentiation mix stimulation)
- Cell Model :
Preadipocytes
- Parameter/biomarker/endpoint :
mRNA: e.g. LPL, FABP4, PPARG, COL1A1...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
TNF-alpha
PREAD-0007 : Preadipocytes, differentiation, lipid content
- Cell Model :
Preadipocytes
- Parameter/biomarker/endpoint :
Lipid labeling
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
Control
TNF-alpha
PREAD-0011 : Preadipocytes, differentiation, lipid droplets formation
- Cell Model :
Preadipocytes
- Parameter/biomarker/endpoint :
Perilipin/lipids/nuclear labeling
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
Control
TNF-alpha
PREAD-0006 : Preadipocytes, differentiation, morphological changes
- Cell Model :
Preadipocytes
- Parameter/biomarker/endpoint :
Morphological changes
- Method :
Microscopy
- Stimulating factor/inducer :
Differentiation mix
- Reference compound :
TNF- alpha
Non-differentiated
Differentiated
