Lipolysis
3T3L1-0001 : 3T3-L1, NEFA release (basal)
- Cell Model :
3T3-L1 preadipocyte cell line (differentiated)
- Parameter/biomarker/endpoint :
Non-esterified fatty acids release
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
Isoproterenol
NEFA release
NEFA release
NHA-0002 : Adipocytes, glycerol release (basal)
- Cell Model :
Hypodermal adipocytes ex vivo
- Parameter/biomarker/endpoint :
Glycerol release
- Method :
Fluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
Theophyllin or isoproterenol
Glycerol release
NHA-0001 : Adipocytes, NEFA release (basal)
- Cell Model :
Hypodermal adipocytes ex vivo
- Parameter/biomarker/endpoint :
Non-esterified fatty acids release
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
Theophyllin or isoproterenol
NEFA release
NHA-0003 : NHA, cAMP content (basal)
- Cell Model :
Hypodermal adipocytes ex vivo
- Parameter/biomarker/endpoint :
cAMP content
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Caffeine or isoproterenol
cAMP content
BIOCH-0013 : Phosphodiesterase inhibition (purified enzyme)
- Cell Model :
Purified type-IV phosphodiesterase
- Parameter/biomarker/endpoint :
Phosphodiesterase activity
- Method :
Radioactivity
- Stimulating factor/inducer :
Basal
- Reference compound :
Caffeine
Phosphodiesterase
PREAD-0010 : Preadipocytes, calcium mobilization
- Cell Model :
Preadipocytes
- Parameter/biomarker/endpoint :
Calcium mobilization
- Method :
Time-lapse microscopy
- Stimulating factor/inducer :
Basal
- Reference compound :
Bradykinin
Calcium mobilization
PREAD-0002 : Preadipocytes, glycerol release (basal)
- Cell Model :
Differentiated preadipocytes
- Parameter/biomarker/endpoint :
Glycerol release
- Method :
Fluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
Isoproterenol
Glycerol release
PREAD-0001 : Preadipocytes, NEFA release (basal)
- Cell Model :
Differentiated preadipocytes
- Parameter/biomarker/endpoint :
Non-esterified fatty acids release
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
Theophyllin or isoproterenol
NEFA release
