Keratinocyte inflammatory response to small bioactive molecules
NCTC-0003 : NCTC 2544, IL-1 alpha release (PMA/A23187/Ca2+ stimulation)
- Cell Model :
NCTC 2544 cell line
- Parameter/biomarker/endpoint :
IL-1 alpha release
- Method :
ELISA
- Stimulating factor/inducer :
PMA+A23187+Ca2+-stimulated
- Reference compound :
Dexamethasone
IL-1alpha
NCTC-0001 : NCTC 2544, IL-8 release (PMA stimulation)
- Cell Model :
NCTC 2544 cell line
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Dexamethasone
IL-8
NCTC-0002 : NCTC 2544, PGE2 release (PMA stimulation)
- Cell Model :
NCTC 2544 cell line
- Parameter/biomarker/endpoint :
PGE2 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Indomethacin
PGE2
NHEK-0048 : NHEK, calcium mobilization (bradykinin stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Calcium mobilization
- Method :
Time-lapse microscopy
- Stimulating factor/inducer :
Bradykinin
Bradykinin-stimulated
Calcium mobilization
NHEK-0032 : NHEK, cAMP content (forskolin stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
cAMP content
- Method :
ELISA
- Stimulating factor/inducer :
Forskolin
cAMP content
NHEK-0040 : NHEK, IL-1 alpha release (PMA/A23187/Ca2+ stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
IL-1 alpha release
- Method :
ELISA
- Stimulating factor/inducer :
PMA+A23187+Ca2+-stimulated
- Reference compound :
Dexamethasone
IL-1alpha
NHEK-0001 : NHEK, IL-8 release (PMA stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Dexamethasone
IL-8
NHEK-0078 : NHEK, IL-8 release (poly(I:C) stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
Poly (I:C)
IL-8
NHEK-0002 : NHEK, PGE2 release (PMA/A23187/Ca2+ stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
PGE2 release (low)
- Method :
ELISA
- Stimulating factor/inducer :
PMA+A23187+Ca2+-stimulated
- Reference compound :
Indomethacin
PGE2
EPIBA-0061 : RHE, gene expression (PMA stimulation)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
mRNA for selected marker(s)
- Method :
RT-qPCR
- Stimulating factor/inducer :
PMA
PMA
