UV, solar, free radicals, sunburn
BIOCH-0009 : Acellular, free radical scavenging
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
ORAC
- Method :
Photometry
BIOCH-0007 : Acellular, free radical scavenging
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
DPPH
- Method :
Photometry
- Reference compound :
alpha-tocopherol
DPPH reduction
BIOCH-0008 : Acellular, free radical scavenging
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
H2O2 measurement (Amplex Red)
- Method :
Fluorescence
- Reference compound :
BHA
H2O2
NHEK-0003 : NHEK, gene expression (UV stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Selection of genes of interest
- Method :
RT-qPCR
- Stimulating factor/inducer :
UVB(+A)
UVB(+A)
UVB(+A)
NHEK-0005 : NHEK, H2O2 content (UV stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
H2O2, DHR probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
BHA
Hydrogen peroxide content
NHEK-0109 : NHEK, JNK activation (H2O2 stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
JNK phosphorylation
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
H2O2
- Reference compound :
SP600125
Control
H2O2
NHEK-0110 : NHEK, JNK activation (UV stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
JNK phosphorylation
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
Vitamin D3
Control
UVB+(A)
NHEK-0006 : NHEK, lipid peroxide content (UV stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Lipid peroxide, C11-Fluor probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
BHA
Lipid peroxide content
EPIBA-0010 : RHE, CPD determination (UV stimulation)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
DNA cyclobutane pyrimidine dimers
- Method :
Immunofluorescence
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
Sun cream
Control
UVB(+A)
EPIBA-0004 : RHE, gene expression (UV stimulation)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Selection of genes of interest
- Method :
RT-qPCR
- Stimulating factor/inducer :
UVB(+A)
UVB(+A)
UVB(+A)
