Intercellular communication
NHEK-0079 : NHEK, intercellular communication, gap-FRAP
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Gap junction intercellular communication
- Method :
Fluorescence / imaging
NHEK-0108 : NHEK, intercellular communication, scrap loading
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Gap junction intercellular communication
- Method :
Fluorescence / imaging
- Reference compound :
Retinoic acid and cadmium chloride
Ctrl
Retinoic acid
NHEK-0081 : NHEK, mRNA for gap junction proteins (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
mRNA: e.g. GJB2, GJA1...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
EPIBA-0041 : RHE, gap junction proteins expression (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Expression of e.g. connexin 26, connexin 43…
- Method :
Western blot
- Stimulating factor/inducer :
Basal
Connexin 26
EPIBA-0042 : RHE, mRNA for gap junction proteins (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
mRNA: e.g. GJB2, GJA1...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
SKIN-0022 : Skin maintained ex vivo, mRNA for gap junction proteins (basal)
- Cell Model :
Skin (ex vivo)
- Parameter/biomarker/endpoint :
mRNA: e.g. GJB2, GJA1...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
