Fibroblast viability & metabolism
DE-0001 : DE, dermis strength and cohesion (basal)
- Cell Model :
Dermis-equivalent
- Parameter/biomarker/endpoint :
Lattices contraction
- Method :
Imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Control
TGF-beta
NHDF-0017 : NHDF, ATP content (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
ATP
- Method :
Luminescence
- Stimulating factor/inducer :
Basal
NHDF-0014 : NHDF, glucose consumption (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Glucose quantification (kinetics)
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
FCS
Glucose consumption
NHDF-0004 : NHDF, migration (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Migration
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
FCS
Control
FCS
NHDF-0015 : NHDF, mitochondrial dehydrogenase activity (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
MTT reduction
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
FCS
Viability
NHDF-0016 : NHDF, mitochondrial morphology and activity (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Mitochondrial morphology & activity; 2 probes
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta + FCS
Mitochondria
Activity
NHDF-0003 : NHDF, proliferation (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Proliferation
- Method :
[3H]-thymidine incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF
NHDF proliferation
NHDF-0019 : NHDF, proliferation (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
BrdU incorporation
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF
NHDF proliferation
NHDF-0018 : NHDF, proteasome activity (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Proteasome activity
- Method :
Fluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
Lactacystin proteasome inhibitor
Proteasome activity
