Melanogenesis
B16-0002 : B16, inhibition of melanin synthesis (MSH-induced, whitening agents)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
Melanin content
- Method :
Photometry
- Stimulating factor/inducer :
NDP-MSH
- Reference compound :
Kojic acid
Melanin content
B16-0016 : B16, pigmentation-related gene expression (basal)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
IBMX
IBMX
B16-0018 : B16, pigmentation-related gene expression (MSH stimulation)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
mRNA: e.g. TYR, TRP1, TRP2, MITF, MC1R,...
- Method :
RT-qPCR
- Stimulating factor/inducer :
NDP-MSH
NDP-MSH
B16-0001 : B16, stimulation of melanin synthesis (basal, pro-pigmenting agents)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
Melanin content
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
NDP-MSH
B16 melanocytes
Melanin content
B16-0012 : B16, TRP-1 expression (basal)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
TRP-1 immunolabeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
IBMX
Control
IBMX
B16-0013 : B16, TRP-1 expression (MSH stimulation)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
TRP-1 expression
- Method :
Immunofluorescence
- Stimulating factor/inducer :
NDP-MSH
Control
NDP-MSH
B16-0014 : B16, tyrosinase activity (basal)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
Tyrosinase activity
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
IBMX or Kojic acid
Tyrosinase activity
B16-0010 : B16, tyrosinase expression (basal)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
Tyrosinase immunolabeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
NDP-MSH or IBMX
Control
IBMX
B16-0011 : B16, tyrosinase expression (MSH stimulation)
- Cell Model :
B16 melanoma cell line
- Parameter/biomarker/endpoint :
Tyrosinase immunolabeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
NDP-MSH
Control
NDP-MSH
FOLL-0009 : Hair, pigmentation-related gene expression (basal)
- Cell Model :
Hair follicle
- Parameter/biomarker/endpoint :
mRNA: e.g. TYR, TRP1, MITF, MC1R,...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
