Photo-aging (dermis)
BIOCH-0019 : Caspase 3/7, enzymatic activity
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
Caspase 3/7 activity
- Method :
Fluorescence
- Stimulating factor/inducer :
Staurosporine
- Reference compound :
Ac-DEVD-CHO
Caspase
Caspase 2
JURK-0001 : Jurkat cells, H2O2 content (UV stimulation)
- Cell Model :
Jurkat cell line
- Parameter/biomarker/endpoint :
H2O2 (cellular), DHR probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
BHA
Hydrogen peroxide content
JURK-0003 : Jurkat cells, lipid peroxide content (UV stimulation)
- Cell Model :
Jurkat cell line
- Parameter/biomarker/endpoint :
Lipid peroxides (cellular), C11-Fluor
- Method :
Flow cytometry
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
BHA
Lipid peroxide content
NHDF-0001 : NHDF, MMP-1 release (UV stimulation)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
MMP-1 release
- Method :
ELISA
- Stimulating factor/inducer :
UVA
- Reference compound :
Dexamethasone
MMP-1
NHDF-0024 : NHDF, caspase 3/7, enzymatic activity (UV stimulation)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Caspase 3/7 activity
- Method :
Fluorescence
- Stimulating factor/inducer :
UV
Caspase
Caspase 2
NHDF-0002 : NHDF, gene expression (UV stimulation)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Selection of genes of interest
- Method :
RT-qPCR
- Stimulating factor/inducer :
UVA
- Reference compound :
Dexamethasone
UVA-stimulated
UVA-stimulated
