Lipogenesis, fat storage
3T3L1-0004 : 3T3-L1, lipid neosynthesis (basal)
- Cell Model :
3T3-L1 preadipocyte cell line (differentiated)
- Parameter/biomarker/endpoint :
Lipid neosynthesis
- Method :
[14C]-acetate incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
Cerulenin
Lipids
3T3L1-0005 : 3T3-L1, lipid neosynthesis (basal)
- Cell Model :
3T3-L1 preadipocyte cell line (differentiated)
- Parameter/biomarker/endpoint :
Lipid neosynthesis
- Method :
[3H]-glucose incorporation
- Stimulating factor/inducer :
Basal
Glucose incorporation
3T3L1-0006 : 3T3-L1, lipid neosynthesis (basal)
- Cell Model :
3T3-L1 preadipocyte cell line (differentiated)
- Parameter/biomarker/endpoint :
Fatty acids uptake
- Method :
[14C]-oleate incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
Cerulenin
Fatty acid uptake
3T3L1-0008 : 3T3-L1, active lipoprotein lipase (LPL) production (basal)
- Cell Model :
3T3-L1 preadipocyte cell line (differentiated)
- Parameter/biomarker/endpoint :
Synthesis / release of active LPL
- Method :
Radioactivity
- Stimulating factor/inducer :
Basal
LPL production
BIOCH-0016 : Lipoprotein lipase (LPL) activity
- Cell Model :
LPL from bovine milk
- Parameter/biomarker/endpoint :
LPL activity
- Method :
Radioactivity
- Stimulating factor/inducer :
Basal
- Reference compound :
Inhibitor cocktail
LPL activity
BIOCH-0026 : Pancreatic lipase activity
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
Non-esterified fatty acids release
- Method :
Photometry
- Stimulating factor/inducer :
Basal
NHA-0004 : Adipocytes, lipid neosynthesis (basal)
- Cell Model :
Hypodermal adipocytes ex vivo
- Parameter/biomarker/endpoint :
Lipid neosynthesis
- Method :
[14C]-acetate incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
Cerulenin or EPA
Lipids
PREAD-0013 : Preadipocytes, active lipoprotein lipase production (LPL) (basal)
- Cell Model :
Differentiated preadipocytes
- Parameter/biomarker/endpoint :
Active LPL synthesis/release
- Method :
Radioactivity
- Stimulating factor/inducer :
Basal
Active LPL
