Protection against infections, skin innate immunity
EPIBA-0048 : RHE, skin innate immunity markers expression (Basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
S100A7 expression
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
S100A7
S100A7
EPIBA-0049 : RHE, IL-8 release (Basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
IL-8
EPIBA-0050 : RHE, antimicrobial peptide gene expression (Basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
Cytokines-stimulated
EPIBA-0060 : RHE, Staphylococcus aureus adhesion (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
S. aureus adhesion
- Method :
Radioactivity
- Stimulating factor/inducer :
Basal
NHEK-0035 : NHEK, beta-defensin 2/4 release (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
beta-defensin 2/4 release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
beta-defensin 2/4
NHEK-0036 : NHEK, S100A7 expression (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
S100A7 immunolabeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
Control
Cytokine mix
NHEK-0037 : NHEK, antimicrobial peptide gene expression (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
Cytokine-stimulated
Cytokine-stimulated
NHEK-0039 : NHEK, IL-8 expression (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
IL-8
SKIN-0033 : Skin maintained ex vivo, Staphylococcus aureus adhesion (basal)
- Cell Model :
Skin (ex vivo)
- Parameter/biomarker/endpoint :
S. aureus adhesion
- Method :
Dot blot
- Stimulating factor/inducer :
Basal
- Reference compound :
Aminoguanidine
