Epidermal matrix, hydration
BIOCH-0005 : Hyaluronidase inhibition (purified enzyme)
- Cell Model :
Purified enzyme
- Parameter/biomarker/endpoint :
Hyaluronidase activity
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
MgCl2
Hyaluronidase activity
EPIBA-0028 : RHE, hyaluronic acid synthesis/release (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Hyaluronic acid synthesis / release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
Hyaluronic acid
EPIBA-0029 : RHE, total GAGs neosynthesis (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Glycosaminoglycans (GAGs, total)
- Method :
[3H]-glucosamine incorporation
- Stimulating factor/inducer :
Basal
EPIBA-0030 : RHE, sulfated GAGs neosynthesis (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Sulfated glycosaminoglycans (sulfated GAGs)
- Method :
[35S]-sulfate incorporation
- Stimulating factor/inducer :
Basal
EPIBA-0031 : RHE, hyaluronic acid expression (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Hyaluronic acid immunolabeling
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
Hyaluronic acid
EPIBA-0032 : RHE, protein markers (PGs, ECM receptors...) expression (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Expression of e.g. CD44, alpha2 beta1 integrin...
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
CD44
EPIBA-0033 : RHE, mRNA for epidermal matrix-related proteins (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
mRNA CD44
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
Retinoic acid
Retinoic acid
NHEK-0066 : NHEK, hyaluronic acid synthesis/release (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Hyaluronic acid synthesis / release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Retinoic acid
Hyaluronic acid
NHEK-0067 : NHEK, total GAGs neosynthesis (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Glycosaminoglycans (GAGs, total)
- Method :
[3H]-glucosamine incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
Calcium
Total GAGs
NHEK-0068 : NHEK, sulfated GAGs neosynthesis (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Sulfated glycosaminoglycans (sulfated GAGs)
- Method :
[35S]-sulfate incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
Calcium
Sulfated GAGs
