Keratinocyte viability & metabolism
EPIBA-0012 : RHE, sirtuin 1 expression (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Sirtuin 1 immunolabeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
Basal
EPIBA-0016 : RHE, mitochondrial dehydrogenase activity (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
MTT reduction
- Method :
Photometry
- Stimulating factor/inducer :
Basal
Cytotoxicity
EPIBA-0017 : RHE, ATP content (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
ATP
- Method :
Luminescence
- Stimulating factor/inducer :
Basal
EPIBA-0056 : RHE, glucose consumption (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Glucose quantification (kinetics)
- Method :
Photometry
- Stimulating factor/inducer :
Basal
Glucose consumption
EPIBA-0059 : RHE, epidermal morphology (Hematoxylin-Eosin staining) (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Method :
Histology (HE staining)
- Stimulating factor/inducer :
Basal
HE staining
NHEK-0004 : NHEK, malate dehydrogenase activity
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
MDH activity
- Method :
Photometry
- Stimulating factor/inducer :
Basal
NHEK-0044 : NHEK, glucose consumption (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Glucose quantification (kinetics)
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
FCS
Glucose consumption
NHEK-0049 : NHEK, mitochondrial dehydrogenase activity (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
MTT reduction
- Method :
Photometry
- Stimulating factor/inducer :
Basal
MTT labeling
NHEK-0050 : NHEK, mitochondrial morphology and activity (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Mitochondrial morphology & activity; 2 probes
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
FCS
Immunofluorescence
Activity
NHEK-0051 : NHEK, ATP content (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
ATP
- Method :
Luminescence
- Stimulating factor/inducer :
Basal
