Proliferation, migration, re-epithelialization
EPI&DE-0001 : Epidermis/Dermis, keratinocyte migration (basal)
- Cell Model :
Normal skin epidermis onto a dermis-equivalent
- Parameter/biomarker/endpoint :
Migration (+proliferation)
- Method :
Imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF + FCS
Control
EGF + FCS
EPIBA-0018 : RHE, Ki67 expression (proliferation marker) (basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
Ki67
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
Ki67
Propidium iodide
NHEK-0033 : NHEK, migration (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Migration
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF or OSM
Control
EGF
NHEK-0053 : NHEK, proliferation (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Proliferation
- Method :
BrdU incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF + PE
Proliferation
NHEK-0057 : NHEK, epsin3 expression (migration marker) (basal)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
Epsin 3 immunolabeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF
Immunofluorescence
Image analysis
SKIBA-0029 : FTSK, Ki67 expression (proliferation marker) (basal)
- Cell Model :
Full thickness reconstructed skin (SKI-Ba)
- Parameter/biomarker/endpoint :
Ki67
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
Ki67
SKIN-0006 : Skin maintained ex vivo, Ki67 expression (proliferation marker) (basal)
- Cell Model :
Skin (ex vivo)
- Parameter/biomarker/endpoint :
Ki67
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
Ki67
Propidium iodide
