ECM degradation
BIOCH-0002 : MMP-1 inhibition (recombinant enzyme)
- Cell Model :
Purified enzyme
- Parameter/biomarker/endpoint :
Human MMP-1 activity
- Method :
Fluorescence
- Reference compound :
TIMP-1
MMP-1 activity
BIOCH-0003 : MMP-9 inhibition (recombinant enzyme)
- Cell Model :
Purified enzyme
- Parameter/biomarker/endpoint :
Human MMP-9 activity
- Method :
Fluorescence
- Reference compound :
TIMP-1
MMP-9 activity
BIOCH-0004 : Whole MMP inhibition (NHDF extracts)
- Cell Model :
Enzyme extract
- Parameter/biomarker/endpoint :
MMP activity
- Method :
Fluorescence
- Reference compound :
O-phenanthroline
Whole MMP activity
BIOCH-0005 : Hyaluronidase inhibition (purified enzyme)
- Cell Model :
Purified enzyme
- Parameter/biomarker/endpoint :
Hyaluronidase activity
- Method :
Photometry
- Stimulating factor/inducer :
Basal
- Reference compound :
MgCl2
Hyaluronidase activity
BIOCH-0006 : Leukocyte elastase inhibition (purified enzyme)
- Cell Model :
Purified enzyme
- Parameter/biomarker/endpoint :
Elastase activity (serine-type enzyme)
- Method :
Fluorescence
- Reference compound :
AAPV
Elastase activity
BIOCH-0021 : Collagenase inhibition (bacterial enzyme)
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
Collagenase activity
- Method :
Fluorescence
- Reference compound :
O-phenanthroline
Collagenase activity
NHDF-0001 : NHDF, MMP-1 release (UV stimulation)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
MMP-1 release
- Method :
ELISA
- Stimulating factor/inducer :
UVA
- Reference compound :
Dexamethasone
MMP-1
NHDF-0057 : NHDF, MMP-1 release (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
MMP-1 release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Dexamethasone
MMP-1
