Intrinsic aging (dermis)
DE-0014 : DE, dermis (containing aged NHDF) strength and cohesion (basal)
- Cell Model :
Dermis-equivalent
- Parameter/biomarker/endpoint :
Lattices contraction
- Method :
Imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Lattice contraction
NHDF-0040 : NHDF, Non-enzymatic ECM cross-linking (basal)
- Cell Model :
Fibroblast-produced extracellular matrix
- Parameter/biomarker/endpoint :
ECM glycation
- Method :
[3H]-glucose incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
Aminoguanidine
Glucose incorporation
NHDF-0041 : NHDF, Non-enzymatic ECM cross-linking (basal)
- Cell Model :
Fibroblast-produced extracellular matrix
- Parameter/biomarker/endpoint :
ECM glycation (AGE detection)
- Method :
Dot blot
- Stimulating factor/inducer :
Basal
- Reference compound :
Aminoguanidine
Advanced glycation end-products
NHDFA1-0001 : Aged NHDF, control of aging, proliferation (basal)
- Cell Model :
Dermal fibroblasts, replicative aging model (Hayflick)
- Parameter/biomarker/endpoint :
Proliferation
- Method :
[3H]-thymidine incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF
Proliferation
NHDFA1-0002 : Aged NHDF, control (reversion) of aging, procollagen I & MMP-1 synthesis (basal)
- Cell Model :
Dermal fibroblasts, replicative aging model (Hayflick)
- Parameter/biomarker/endpoint :
Procollagen I synthesis / release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Procollagen I
MMP-1
NHDFA1-0004 : Aged NHDF, control (reversion) of aging (basal)
- Cell Model :
Dermal fibroblasts, replicative aging model (Hayflick)
- Parameter/biomarker/endpoint :
Aging markers
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
TGF-beta
NHDFA1-0005 : Aged NHDF, control (reversion) of aging (basal)
- Cell Model :
Dermal fibroblasts, replicative aging model (Hayflick)
- Parameter/biomarker/endpoint :
beta-galactosidase activity
- Method :
Microscopy
- Reference compound :
TGF- beta
Normal NHDF
Aged NHDF
NHDFA1-0007 : Aged NHDF, control of aging, migration (basal)
- Cell Model :
Dermal fibroblasts, replicative aging model (Hayflick)
- Parameter/biomarker/endpoint :
Migration
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
FCS
Normal NHDF
Aged NHDF
NHDFA1-0008 : Aged NHDF, control (reversion) of aging, hyaluronic acid synthesis (basal)
- Cell Model :
Dermal fibroblasts, replicative aging model (Hayflick)
- Parameter/biomarker/endpoint :
Hyaluronic acid synthesis / release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Hyaluronic acid
Hyaluronic acid
NHDFA1-0009 : Aged NHDF, control (reversion) of aging, total GAGs synthesis (basal)
- Cell Model :
Dermal fibroblasts
- Parameter/biomarker/endpoint :
Glycosaminoglycans (GAGs, total)
- Method :
[3H]-glucosamine incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Total GAGs synthesis
Total GAGs synthesis
