Miscellaneous free radicals assays
BIOCH-0007 : Acellular, free radical scavenging
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
DPPH
- Method :
Photometry
- Reference compound :
alpha-tocopherol
DPPH reduction
BIOCH-0008 : Acellular, free radical scavenging
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
H2O2 measurement (Amplex Red)
- Method :
Fluorescence
- Reference compound :
BHA
H2O2
BIOCH-0009 : Acellular, free radical scavenging
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
ORAC
- Method :
Photometry
BIOCH-0025 : TBARS formation
- Cell Model :
Acellular
- Parameter/biomarker/endpoint :
TBARS production
- Method :
Photometry
- Stimulating factor/inducer :
FeSO4
- Reference compound :
Trolox
TBARS formation
JURK-0001 : Jurkat cells, H2O2 content (UV stimulation)
- Cell Model :
Jurkat cell line
- Parameter/biomarker/endpoint :
H2O2 (cellular), DHR probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
BHA
Hydrogen peroxide content
JURK-0003 : Jurkat cells, lipid peroxide content (UV stimulation)
- Cell Model :
Jurkat cell line
- Parameter/biomarker/endpoint :
Lipid peroxides (cellular), C11-Fluor
- Method :
Flow cytometry
- Stimulating factor/inducer :
UVB(+A)
- Reference compound :
BHA
Lipid peroxide content
NHEK-0109 : NHEK, JNK activation (H2O2 stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
JNK phosphorylation
- Method :
Fluorescence / imaging
- Stimulating factor/inducer :
H2O2
- Reference compound :
SP600125
Control
H2O2
