Atopic dermatitis
CD4TL-0005 : CD4+ T cells, cytokine release (anti-CD3/anti-CD28 stimulation)
- Cell Model :
CD4+ T cells
- Parameter/biomarker/endpoint :
IL-4 release
- Method :
ELISA
- Stimulating factor/inducer :
Anti-CD3/anti-CD28
- Reference compound :
Cyclosporin A
IL-4
NHEK-0038 : NHEK, gene expression (poly (I:C) + Th2 cytokines stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
qPCRarray (16 genes)
- Method :
RT-qPCR
- Stimulating factor/inducer :
Poly (I:C) + Th2 cytokines
- Reference compound :
NF kappaB inhibitor
Poly (I:C) + Th2
NHEK-0090 : NHEK, gene expression (poly (I:C) + Th2 cytokines stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
mRNA TSLP
- Method :
RT-qPCR
- Stimulating factor/inducer :
Poly (I:C) + Th2 cytokines
- Reference compound :
NF kappaB inhibitor
TSLP
Poly(I:C) + Th2
NHEK-0091 : NHEK, TSLP release (poly (I:C) + Th2 cytokines stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
TSLP release
- Method :
ELISA
- Stimulating factor/inducer :
Poly (I:C) + Th2 cytokines
- Reference compound :
NF kappaB inhibitor
TSLP
PBMC-0009 : T cells, cytokine release (anti-CD3/anti-CD28 stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
IL-4 release
- Method :
ELISA
- Stimulating factor/inducer :
Anti-CD3/anti-CD28
- Reference compound :
Cyclosporin A
IL-4
