Keratinocyte response to small bioactive molecules
EPIBA-0001 : RHE, IL-8 release (PMA stimulation)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Dexamethasone
Reconstructed epidermis
IL-8
EPIBA-0002 : RHE, PGE2 release (PMA stimulation)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
PGE2 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Indomethacin
Reconstructed epidermis
PGE2
EPIBA-0061 : RHE, gene expression (PMA stimulation)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
mRNA for selected marker(s)
- Method :
RT-qPCR
- Stimulating factor/inducer :
PMA
PMA
NCTC-0001 : NCTC 2544, IL-8 release (PMA stimulation)
- Cell Model :
NCTC 2544 cell line
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Dexamethasone
IL-8
NCTC-0002 : NCTC 2544, PGE2 release (PMA stimulation)
- Cell Model :
NCTC 2544 cell line
- Parameter/biomarker/endpoint :
PGE2 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Indomethacin
PGE2
NCTC-0003 : NCTC 2544, IL-1 alpha release (PMA/A23187/Ca2+ stimulation)
- Cell Model :
NCTC 2544 cell line
- Parameter/biomarker/endpoint :
IL-1 alpha release
- Method :
ELISA
- Stimulating factor/inducer :
PMA+A23187+Ca2+-stimulated
- Reference compound :
Dexamethasone
IL-1alpha
NHEK-0001 : NHEK, IL-8 release (PMA stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Dexamethasone
IL-8
NHEK-0002 : NHEK, PGE2 release (PMA/A23187/Ca2+ stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
PGE2 release (low)
- Method :
ELISA
- Stimulating factor/inducer :
PMA+A23187+Ca2+-stimulated
- Reference compound :
Indomethacin
PGE2
NHEK-0032 : NHEK, cAMP content (forskolin stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
cAMP content
- Method :
ELISA
- Stimulating factor/inducer :
Forskolin
cAMP content
NHEK-0040 : NHEK, IL-1 alpha release (PMA/A23187/Ca2+ stimulation)
- Cell Model :
Epidermal keratinocytes
- Parameter/biomarker/endpoint :
IL-1 alpha release
- Method :
ELISA
- Stimulating factor/inducer :
PMA+A23187+Ca2+-stimulated
- Reference compound :
Dexamethasone
IL-1alpha
