Wound healing, trauma & agressions
DE-0006 : DE, hyaluronic acid synthesis/release (basal)
- Cell Model :
Dermis-equivalent
- Parameter/biomarker/endpoint :
Hyaluronic acid synthesis / release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Hyaluronic acid
DE-0008 : DE, total GAGs neosynthesis (basal)
- Cell Model :
Dermis-equivalent
- Parameter/biomarker/endpoint :
Glycosaminoglycans (GAGs, total)
- Method :
[3H]-glucosamine incorporation
- Stimulating factor/inducer :
Basal
- Reference compound :
TGF- beta
Total GAGs synthesis
Supernatants
DRG-0001 : DRG network (basal)
- Cell Model :
Dorsal root ganglia
- Parameter/biomarker/endpoint :
NF-H labeling
- Method :
Immunofluorescence
- Stimulating factor/inducer :
Basal
- Reference compound :
NGF
Control
NGF-stimulated
EPI&DE-0001 : Epidermis/Dermis, keratinocyte migration (basal)
- Cell Model :
Normal skin epidermis onto a dermis-equivalent
- Parameter/biomarker/endpoint :
Migration (+proliferation)
- Method :
Imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
EGF + FCS
Control
EGF + FCS
EPIBA-0048 : RHE, skin innate immunity markers expression (Basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
S100A7 expression
- Method :
Immunofluorescence or IHC
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
S100A7
S100A7
EPIBA-0049 : RHE, IL-8 release (Basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
IL-8
EPIBA-0050 : RHE, antimicrobial peptide gene expression (Basal)
- Cell Model :
Reconstructed epidermis (EPI-Ba)
- Parameter/biomarker/endpoint :
mRNA: e.g. DEFB4, S100A7, PI3, LL37, RNASE7,...
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
Cytokine mix
Cytokines-stimulated
HMVEC-0001 : HMVEC, migration (basal)
- Cell Model :
Dermal microvascular endothelial cells
- Parameter/biomarker/endpoint :
Migration
- Method :
Boyden chamber
- Stimulating factor/inducer :
Basal
- Reference compound :
VEGF
HMVEC migration
HMVEC-0003 : HMVEC, differentiation (basal)
- Cell Model :
Dermal microvascular endothelial cells
- Parameter/biomarker/endpoint :
Pseudotube formation
- Method :
Imaging
- Stimulating factor/inducer :
Basal
- Reference compound :
Suramine
Control
Suramine
HMVEC-0006 : HMVEC, migration (VEGF stimulation)
- Cell Model :
Dermal microvascular endothelial cells
- Parameter/biomarker/endpoint :
Migration
- Method :
Boyden chamber
- Stimulating factor/inducer :
VEGF
HMVEC migration
