Monocytes/macrophages: inflammation, innate immunity
HL60-0001 : HL-60, IL-8 release (PMA stimulation)
- Cell Model :
HL-60 (vitD3/TGF- beta-differentiated)
- Parameter/biomarker/endpoint :
IL-8 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Dexamethasone
IL-8
HL60-0002 : HL-60, arachidonic acid metabolite release (A23187 stimulation)
- Cell Model :
HL-60 (vitD3/TGF- beta-differentiated)
- Parameter/biomarker/endpoint :
LTB4 release
- Method :
ELISA
- Stimulating factor/inducer :
Ionophore A23187
- Reference compound :
AA861
LTB4
PGE2
HL60-0003 : HL-60, CAMP (LL-37 precursor) mRNA expression (basal)
- Cell Model :
HL-60 cell line
- Parameter/biomarker/endpoint :
Cathelicidin / LL-37 expression
- Method :
RT-qPCR
- Stimulating factor/inducer :
Basal
- Reference compound :
Vitamin D3
LL-37 mRNA expression
MONO-0001 : CD14+ monocytes, cytokine release (LPS stimulation)
- Cell Model :
CD14+ monocytes
- Parameter/biomarker/endpoint :
TNF- alpha release
- Method :
ELISA
- Stimulating factor/inducer :
LPS
- Reference compound :
Dexamethasone
IL-1beta
MONO-0002 : CD14+ monocytes, cytokine release (LPS stimulation)
- Cell Model :
CD14+ monocytes
- Parameter/biomarker/endpoint :
IL-1 beta release
- Method :
Flow cytometry (CBA: human inflammatory cytokine kit)
- Stimulating factor/inducer :
LPS
- Reference compound :
Dexamethasone
PBMC-0005 : Monocytes, cytokine release (LPS stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
TNF- alpha release
- Method :
ELISA
- Stimulating factor/inducer :
LPS
- Reference compound :
Dexamethasone
IL-1 beta
PBMC-0006 : PBMC, H2O2 content (PMA stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
H2O2 (cellular), DHR probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
PMA
- Reference compound :
BHA
Hydrogen peroxide content
PBMC-0007 : PBMC, O2- content (PMA stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
O2- (cellular), Hydroxyethidine probe
- Method :
Flow cytometry
- Stimulating factor/inducer :
PMA
- Reference compound :
DPI
Superoxide anion radical content
PBMC-0013 : Monocytes, multiplex cytokine release (LPS stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
Multiplex cytokines
- Method :
Flow cytometry (CBA: human inflammatory cytokine kit)
- Stimulating factor/inducer :
LPS
- Reference compound :
Dexamethasone
PBMC-0032 : PBMC, PGE2 release (PMA stimulation)
- Cell Model :
Peripheral blood mononuclear cells
- Parameter/biomarker/endpoint :
PGE2 release
- Method :
ELISA
- Stimulating factor/inducer :
PMA
- Reference compound :
Indomethacin
