Ensembles articles, publication et posters de Bioalternatives traitant plus particulièrement de la cicatrisation cutanée et des modèles in vitro et ex vitro développés

Cicatrisation cutanée, phases de granulation et maturation

La troisième phase de cicatrisation consiste à remplacer la matrice de fibrine provisoire par le tissu de granulation. Elle se déroule en plusieurs étapes: la réépithélialisation, la fibroplasie, le dépôt de collagène et l’angiogenèse.
cicatrisation inflammation

Cicatrisation cutanée, hémostase et phase inflammatoire

La phase hémostasique est la réponse immédiate à la blessure, pour stopper la perte de sang au niveau de la plaie dans les premières minutes qui suivent la lésion. Les dommages aux capillaires sanguins et l’hémorragie entraînent une vasoconstriction et la coagulation du sang.

Cicatrisation cutanée, généralités

La cicatrisation cutanée est un processus naturel, dynamique et complexe impliquant de nombreux acteurs pour restaurer les structures cellulaires et tissulaires de la peau : cellules souches, cellules différenciées, follicules pileux, matrice extracellulaire (ECM), protéines, cytokines, certains microARN, ainsi que les systèmes vasculaires et nerveux et des forces mécaniques.

Study of proliferation and 3D epidermal reconstruction from foreskin, auricular and trunk keratinocytes in children

Our studies highlight the potential of foreskin tissue for autograft applications in boys. A suitable alternative donor site for autologous cell transplantation in female paediatric burn patients remains an open question in our department. We tested the hypothesis that in vitro studies and RHE reconstructive capacities of cells from different body sites can be helpful to select an optimal site for keratinocyte isolation before considering graft protocols for girls.

Epidermal healing in burns: autologous keratinocyte transplantation as a standard procedure: update and perspective

In the contexte of skin graft, cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.

Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. in vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.

Quantitative and qualitative study in keratinocytes from foreskin in children: Perspective application in paediatric burns

Keratinocytes from foreskin have a high capacity for division. A potential source of cells to provide coverage in paediatric burns.

Human embryonic stem-cell derivatives for full reconstruction of the pluristratified epidermis: a preclinical study

To assess whether the keratinocyte progeny of human embryonic stem cells (hESCs) could be used to form a temporary skin substitute for use in patients awaiting autologous grafts, we investigated the cells' capability of constructing a pluristratified epidermis.
burns

Cultured keratinocyte cells from foreskin and future application for burns in children

The keratinocytes resulting from foreskin have a high capacity of division. These cells can divide a long time before differentiation. The observations enable us to propose with our patients the keratinocytes from foreskin for wound healing especially for burns in children.
lipidocolloid dressing

Stimulation of the proliferation of human dermal fibroblasts in vitro by a lipidocolloid dressing

The effect of Urgotul on normal human dermal fibroblast proliferation was studied in vitro and compared with that of two other dressing: Mepitel and Tulle Gras.