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2D & 3D in vitro canine models

Evaluation of the effect of a reference molecule on an Atopic Reconstructed Canine Epidermis model (RCE-AD)

Bioalternatives has several canine in vitro models which were initially developed in cooperation with veterinary practitioners, especially in respect to sample sourcing for cell culture.

We have also developed models and protocols suitable for modeling pathologies (inflammation, infections, senescence, degeneration, atopic dermatitis) or aggressions (stress, wound healing).

We are presenting here some results from a study of an atopic reconstructed canine epidermis model. Please, do not hesitate to contact us for more information.

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Objective of the study using atopic reconstructed canine epidermis

The objective of this study is to evaluate the efficacy of a reference molecule (REF) on a model of canine reconstructed atopic epidermis (RCE-AD). In this RCE model, atopic dermatitis is induced through a cocktail of cytokines.

1 – Evaluation of the morphology

Analysis of the morphology by histological staining highlights an inhibitory effect of the reference on the pro-inflammatory effect of the cytokine cocktail (decrease of the spongiosis, increase of the keratohyaline grains).

Histological morphological analysis hemalun eosin staining

2 – Evaluation of the skin barrier permeability

The analysis of the permeability of the membrane is carried out by the quantification of ceramides by LC / MS and by the evaluation of the penetration of the Lucifer yellow within the corneous layer.

The quantification of ceramides by LC / MS shows that the quantity of ceramides decreases in the presence of cytokines and that the reference does not limit the effect of cytokines.

The evaluation of the penetration of Lucifer yellow shows that the presence of cytokines (RCE-AD) increases the permeability of the tissue and promotes the penetration of Lucifer yellow. The reference inhibits the effect of cytokines on the epidermal barrier permeability.

Evaluation of the penetration of Lucifer yellow into the RCE

3 – Analysis of differentiation, adhesion and tight junction proteins

The skin barrier function is also evaluated by analyzing the expression of epidermal and tight junction proteins. Differentiation proteins (Filaggrin) and adhesion proteins (Corneodesmosin) are analyzed by WES (Automated Western Blot). Tight junctions are quantified by immunolabeling.

The evaluation of epidermal proteins and tight junctions shows that the cytokine cocktail (RCE-AD) strongly decreases the expression of profilaggrine, Corneodesmosine and Claudin-1. The reference significantly decreases the effect of cytokines on the expression of these proteins.

Profilaggrin expression

Evaluation of Corneodesmosin expression – WES

Evaluation of Claudin-1 expression by immunolabeling

4 – Evaluation of inflammation

The inflammation is evaluated by ELISA quantification of IL-8 in the medium. The assay reveals a strong increase of IL-8 secretion in the presence of cytokines and a decrease of this inflammation with the reference.

Evaluation of inflammation by ELISA quantification of IL-8

Do you need more information regarding this study? Do you want to evaluate the efficacy of your products on RCE?
Let’s talk about it!

CONTACT US

See also

Effets sur les signes cliniques, les lipides et les facteurs d'hydratation des applications combinées de shampooing et de mousse contenant de l'Ophytrium et du Seboliance chez les chiens atteints de séborrhée
27 November 2020

Effets sur les signes cliniques, les lipides et les facteurs d’hydratation des applications combinées de shampooing et de mousse contenant de l’Ophytrium et du Seboliance chez les chiens atteints de séborrhée

Evaluation of mean inhibition - canine whole bool model
23 July 2020

Évaluation des effets anti-inflammatoires et immunomodulateurs d’un produit sur un modèle in vitro de sang total canin

Modèles canins in vitro
16 July 2020

Modèles canins in vitro 2D et 3D

16 July 2020

Évaluation de l‘effet d’une molécule de référence sur un modèle d’Épiderme Canin Reconstruit atopique (RCE-AD)

25 May 2020

A prospective, randomized, double blind, placebo-controlled evaluation of the effects of an n-3 essential fatty acids supplement (Agepi® ω3) on clinical signs, and fatty acid concentrations in the erythrocyte membrane, hair shafts and skin surface of dogs with poor quality coats

 

 

 

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Bioalternatives, Inc.
250 Park Avenue, 7th Floor,
New York, NY, 10177, USA
Tel. +1 (212) 364 5115
Mail: info.us[@]bioalternatives.com

Bioalternatives SASU
1bis rue des plantes - CS 50011
86160 Gençay - France
Tel. +33 (0) 5 49 36 11 37
Mail: info[@]bioalternatives.com

Latest posts

  • Effects on clinical signs, lipids and hydration factors of combined applications of shampoo and mousse containing Ophytrium and Seboliance in seborrheic dogs
  • Metrology and sensors as dermo-cosmetic technology opportunities for a change of paradigm
  • Evaluation of the anti-inflammatory and immunomodulatory effects of a product using in vitro canine whole blood models

Cosmetic

  • Hydration and skin barrier
  • Epidermal regeneration
  • Skin firmness & cohesion
  • Skin ageing
  • Skin protection & defense
  • Skin pigmentation
  • Oily skin and hyperseborrhea
  • Skin microcirculation and vascularization
  • Slimming and adipocyte metabolism
  • Hair growth and alopecia

Pharmacology

  • Immuno-Inflammation
  • Neurobiology
  • Veterinary medicine

Dermatology

  • Acne
  • Hair growth and alopecia
  • Atopic dermatitis
  • Psoriasis
  • Wound healing and skin regeneration
  • Melanoma
  • Vitiligo (coming soon)
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