Effect of C-xyloside on morphogenesis of the dermal epidermal junction in aged female skin. An ultrastuctural pilot study

European Journal of Dermatology, 21(2):191-6


L’Oréal Recherche, 188, rue Paul Hochart, BP 553, 94152 Chevilly-Larue, France.
L’Oréal Recherche, Clichy, France.
L’Oréal Recherche, Aulnay-sous-Bois, France.
Bioalternatives, Gençay, France.


A placebo-controlled randomized pilot study was performed on five postmenopausal women aged from 60 to 75 years. The women applied 320 mg (2 mg/cm(2)) of either placebo or 10% C-β-D-xylopyranoside-2-hydroxy-propane (C-xyloside) cream to each outer forearm twice daily for 3 months. At the end of the treatment, skin biopsies were collected from application areas on both forearms. Transmission electron microscope examinations revealed skin ultrastructural changes at the dermal epidermal junction (DEJ) after 10% C-xyloside application for 3 months. The morphological appearance of the DEJ showed strong improvements, with more homogeneous and regular lamina densa in the C-xyloside-treated compared to the placebo treated skin areas. The number of zones showing basement membrane re-duplication was indeed strikingly reduced on C-xyloside-treated skin. These ultrastructural results were further confirmed by a statistically significant increase in the expression levels of α6-integrin the and laminin-332, as estimated by immunohistochemistry.
Altogether, these data suggest that topical C-xyloside application in vivo may be efficient in inducing a better dermal-epidermal cohesion when such a junction is deficient, as is the case in photo-aged or chronologically aged skin. Moreover, a statistically significant increase in CD44 expression was noted in the epidermis of C-xyloside-treated compared to the placebo treated skin areas.

KEYWORDS: Skin ageing; Dermal epidermal junction; C-xyloside; Laminin 332; α 6-integrin subunit

Check out Bioalternatives’ updates and experience new testing ideas

  • Bioassays, models and services
  • Posts and publications
  • Events

Related Posts or publications

New Biological Activities of Lythrum salicaria L. The perennial and widespread herb Lythrum salicaria L., also called purple loosestrife, is a plant that is traditionally used in European medicine.
Effects of different kinds of pollutants on the lipidic metabolism of human sebocytes and protective effects of a new macroalgae culture extract Sebocytes lipid production are stimulated by pollutants. Ame acts by protecting epidermal cells, human sebocytes and skin from urban dust.
Acne vulgaris: physiopathology and cell mechanisms Acne Vulgaris is a chronic inflammatory pathology located in the region of the pilosebaceous follicle. It occurs following a high production of sebum linked to hormonal (androgynous) or environmental (pollution) malfunctioning and to the colonization...
Physiology and functions of the sebaceous gland The sebaceous gland is an organ located in the dermis. Its role is to synthesise and secrete sebum which is a component of the hydrolipidic film. The purpose of sebum is to protect the skin from external aggression and dehydration. Sebum also maintai...
Hair: Follicle, Associated Structures and Growth Hair follicles are skin adnexa which are able to regenerate in a complete, autonomous, cyclic and asynchronous manner. The histological analysis of the pilosebaceous unit reveals a three-element organization: the epithelial compartment, the mesenchym...