The conversion of testosterone to 5-dihydrotestosterone (DHT), the active metabolite of testosterone, by the 5α-reductase enzyme is one of the causes of sebaceous gland hypersecretion and of premature hair loss.
The purpose of this assay is to evaluate the effects of compounds (molecules, active ingredients, extracts, etc.) on the 5-α reductase activity of human hair follicle dermal papilla fibroblasts (HFDPC). This assay is based on the incorporation of [14C]-testosterone and on the extraction and separation/radiodetection of the formed metabolites. In particular, it allows the production of the active metabolite of testosterone (dihydrotestosterone) to be measured.