Hyaluronic acid, a sulfate-free glycosaminoglycan, is one of the major components of the extracellular matrix. Hyaluronic acid fills the inter-cellular spaces in skin. It plays an important role in maintaining skin hydration and turgescence, due to its capacity to capture water molecules and ions.
The purpose of this in vitro assay is to evaluate, via an ELISA test, the stimulating effects of compounds (molecules, active ingredients, extracts, etc.) on hyaluronic acid production (synthesis and release) by normal human epidermal keratinocytes.